Increased activity of enzymatic transacylation of acrylates through rational design of lipases

…, HW Hoeffken, C Branneby, S Maurer, B Hauer…

文献索引：Syren, Per-Olof; Lindgren, Ebba; Hoeffken, Hans Wolfgang; Branneby, Cecilia; Maurer, Steffen; Hauer, Bernhard; Hult, Karl Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 65, # 1-4 p. 3 - 10

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被引用次数: 20

摘要

A rational design approach was used to create the mutant Candida antarctica lipase B (CALB, also known as Pseudozyma antarctica lipase B) V190A having a kcat three times higher compared to that of the wild type (wt) enzyme for the transacylation of the industrially important compound methyl methacrylate. The enzymatic contribution to the transacylation of various acrylates and corresponding saturated esters was evaluated by comparing the ...