Using Bioluminescence Turn-On To Detect Cysteine in Vitro and in Vivo
Miaomiao Zhang, Lin Wang, Yangyang Zhao, Fuqiang Wang, Jindao Wu, Gaolin Liang
文献索引：10.1021/acs.analchem.8b00682
全文：HTML全文
摘要
Cysteine (Cys) is an essential amino acid and plays important roles in many biological processes. Bioluminescence (BL) is advantageous in sensitivity but BL probes that were intentionally developed for the selective detection of Cys were rarely reported. Herein, employing a fast conjugate addition between Cys and acrylic ester, we synthesized a caged BL probe acrylic ester luciferin (1) and used it to selectively detect Cys in vitro and image Cys in living cells and in tumor sites. We envision that, in the future, probe 1 might be used for evaluating the Cys roles in more biological processes.