Synthesis of deoxyoligonucleotide linker fragments for genetic engineering using improved preparative and analytical techniques

H Seliger, TC Bach, G Siewert, W Boidol…

文献索引：Seliger, Hartmut; Bach, Trung-Chinh; Siewert, Gerhard; Boidol, Werner; Toepert, Michael; et al. Liebigs Annalen der Chemie, 1984 , # 5 p. 835 - 853

全文：HTML全文

被引用次数: 3

摘要

Abstract For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level. Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins. The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks. ...