Stereospecificity of the reaction catalyzed by enoyl-CoA hydratase

…, X He, HA Hofstein, DP Raleigh, PJ Tonge

文献索引：Wu, Wen-Jin; Feng, Yuguo; He, Xiang; Hofstein, Hilary A.; Raleigh, Daniel P.; Tonge, Peter J. Journal of the American Chemical Society, 2000 , vol. 122, # 17 p. 3987 - 3994

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被引用次数: 26

摘要

Enoyl-CoA hydratase catalyzes the stereospecific hydration of α, β-unsaturated acyl-CoA thiolesters. Hydration of trans-2-crotonyl-CoA to 3 (S)-hydroxybutyryl-CoA proceeds via the syn addition of water and thus the pro-2 R proton of 3 (S)-hydroxybutyryl-CoA is derived from solvent. Incubation of 3 (S)-hydroxybutyryl-CoA with enzyme in D2O results in the slow exchange of the pro-2 S proton with solvent deuterium, in addition to the anticipated rapid ...