Cross-linked crystals of subtilisin: versatile catalyst for organic synthesis

…, K Yakovlevsky, B Zhang, AL Margolin

文献索引：Wang, Yi-Fong; Yakovlevsky, Kirill; Zhang, Bailing; Margolin, Alexey L. Journal of Organic Chemistry, 1997 , vol. 62, # 11 p. 3488 - 3495

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被引用次数: 73

摘要

Cross-linked enzyme crystals (CLECs) of subtilisin exhibit excellent activity in aqueous and various organic solvents. This catalyst is more stable than the native enzyme in both aqueous and mixed aqueous/organic solutions. Subtilisin-CLEC was shown to be a versatile catalyst. It was used for the syntheses of peptides and peptidomimetics, mild hydrolysis of amino acid and peptide amides, enantio-and regioselective reactions, and ...