Exploration of the biocatalytic potential of a halohydrin dehalogenase using chromogenic substrates

JHL Spelberg, L Tang, M van Gelder, RM Kellogg…

文献索引：Lutje Spelberg, Jeffrey H.; Tang, Lixia; Van Gelder, Marc; Kellogg, Richard M.; Janssen, Dick B. Tetrahedron Asymmetry, 2002 , vol. 13, # 10 p. 1083 - 1089

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被引用次数: 80

摘要

Halohydrin dehalogenases are bacterial enzymes that catalyse the reversible formation of epoxides from vicinal halohydrins. A spectrophotometric assay for halohydrin dehalogenases based on the absorption difference between the halohydrin para-nitro-2- bromo-1-phenylethanol and the epoxide para-nitrostyrene oxide was developed. The enantioselectivity of ring-closure reactions catalysed by three different halohydrin ...