Enantioselective Epoxidation of Terminal Alkenes to (R)??and (S)??Epoxides by Engineered Cytochromes P450 BM??3

…, MW Peters, P Meinhold, FH Arnold

文献索引：Kubo, Takafumi; Peters, Matthew W.; Meinhold, Peter; Arnold, Frances H. Chemistry - A European Journal, 2006 , vol. 12, # 4 p. 1216 - 1220

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被引用次数: 112

摘要

Abstract Cytochrome P450 BM-3 from Bacillus megaterium was engineered for enantioselective epoxidation of simple terminal alkenes. Screening saturation mutagenesis libraries, in which mutations were introduced in the active site of an engineered P450, followed by recombination of beneficial mutations generated two P450 BM-3 variants that convert a range of terminal alkenes to either (R)-or (S)-epoxide (up to 83% ee) with high ...