Tetrahedron letters

Incorporation of a fluorescent nucleotide into oligoribonucleotides

CJ Adams, JB Murray, JRP Arnold, PG Stockley

文献索引：Adams, Chris J.; Murray, James B.; Arnold, John R.P.; Stockley, Peter G. Tetrahedron Letters, 1994 , vol. 35, # 10 p. 1597 - 1600

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被引用次数: 25

摘要

Abstract The fluorescent nucleoside 2-pyrimidinone-1-β-d-riboside (4H C) has been incorporated into oligoribonucleotides using standard cyanoethyl phosphoramidite methods. This base provides a useful probe for the exocyclic amino function in cytidine. Cleavage of hammerhead ribozymes using GCGCCGAAACACCGUGUCUCGAGC as the substrate and GGCUCGA [4H C] UGAUGAGGCGC as a modified ribozyme resulted in a cleavage rate ...