Synthesis and utility of sulfated chromogenic carbohydrate model substrates for measuring activities of mucin-desulfating enzymes

…, PL Rhodes, AM Roberton, DI Rosendale, PC Tyler…

文献索引：Clinch, Keith; Evans, Gary B; Furneaux, Richard H; Rendle, Phillip M; Rhodes, Phillippa L; Roberton, Anthony M; Rosendale, Douglas I; Tyler, Peter C; Wright, Damian P Carbohydrate Research, 2002 , vol. 337, # 12 p. 1095 - 1111

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被引用次数: 30

摘要

A chromogenic substrate, 4-nitrophenyl 2-acetamido-2-deoxy-β-d-glucopyranoside 6- sodium sulfate was synthesized and used in combination with β-N-acetylhexosaminidase for detection of the sulfatase, MdsA, by release of 4-nitrophenol. MdsA was originally isolated from the bacterium Prevotella strain RS2 and is believed to be involved in desulfation of sulfomucins, major components of the mucus barrier protecting the human colon surface. ...