149697-16-5

149697-16-5 structure

Name: Z-Ala-Ala-Asn-AMC
Chemical Name: z-ala-ala-asn-amc
CAS Number: 149697-16-5
Molecular Formula: C₂₈H₃₀N₄O₉
Molecular Weight: 565.57400
Catalog: Signaling Pathways Others Others
Create Date: 2017-04-20 02:07:43
Modify Date: 2024-01-08 12:33:01
Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC[1].

Name	z-ala-ala-asn-amc
Synonyms	z-ala-ala-asn-mca benzyloxycarbonyl-l-alanyl-l-alanyl-l-asparagine 4-methylcoumaryl-7-amide z-aan-amc

Description	Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Others >> Others
In Vitro	The first legumain fluorogenic substrate Cbz-Ala-Ala-Asn-AMC based on the P3-P2-P1 sequence Ala-Ala-Asn. The synthetic substrate Cbz-Ala-Ala-Asn-AMC is effectively cleaved by S. mansoni legumain and human legumain with Kms of 90 and 80 uM respectively[2][3].
References	[1]. Stern L, Perry R, Ofek P, Many A, Shabat D, Satchi-Fainaro R. A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain. Bioconjug Chem. 2009;20(3):500-510. [2]. James C. Powers, et al. Propenoyl hydrazides. US7482379B2. [3]. Poreba M, et al. Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes. Cell Chem Biol. 2016;23(8):1023-1035.