10205-62-6
10205-62-6 structure
- Name: BTA-2
- Chemical Name: N,N-dimethyl-4-(6-methyl-1,3-benzothiazol-2-yl)aniline
- CAS Number: 10205-62-6
- Molecular Formula: C16H16N2S
- Molecular Weight: 268.37700
- Catalog: Research Areas Others
- Create Date: 2018-12-31 12:07:35
- Modify Date: 2024-01-02 13:30:27
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BTA-2, a benzothiazole dye, is structurally similar to thioflavin T (ThT), which exhibits an enhanced fluorescence signal when bound to amyloid fibrils. BTA-2 has distinct absorption and emission characteristics in solution and when bound to amyloid fibrils, which makes it can used for identifying amyloid fibrils using spectroscopy[1].
| Name | N,N-dimethyl-4-(6-methyl-1,3-benzothiazol-2-yl)aniline |
|---|---|
| Synonyms |
2-<4-Dimethylamino-phenyl>-6-methyl-benzothiazol
MFCD00192069 EINECS 233-506-7 |
| Description | BTA-2, a benzothiazole dye, is structurally similar to thioflavin T (ThT), which exhibits an enhanced fluorescence signal when bound to amyloid fibrils. BTA-2 has distinct absorption and emission characteristics in solution and when bound to amyloid fibrils, which makes it can used for identifying amyloid fibrils using spectroscopy[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). To research how CH3CN affects binding of amyloid fibrils (varied the amount of CH3CN but maintained the same concentration of BTA-2, fibrils, and water) and how the concentration of BTA-2 affects binding (varied the amount of BTA-2 but had a constant amount of CH3CN, fibrils, and water) [1] . BTA-2 in solution and in the presence of amyloid fibrils: 1. Insulin obtains from a bovine pancreas, and is stored at −20°C in a desiccator. 2. To synthesize BTA-2 and further purified. 3. To prepare fibrils: Insulin is dissolved in pH 2 water (5 mg/mL) and filters through a 0.2 μm filter, heats at 60℃ for 24 h. Centrifuge at 3000 rpm for 2.5 min to remove any globular artifacts. The supernatant containing the fibrils is saved for later use. Solutions are stored in a refrigerator (10℃) until needed. 4. BTA-2/fibrils solutions (are prepared in two ways): (First way) Diluting (100 μL) of the stock fibril solution with 880 μL of pH 2 water, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2 in CH3CN. (Second way) Diluting (100 μL) of the stock fibril solution with a 1:1 mixture of nanopure water: CH3CN, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2. 5. Two similar solutions are made that contained only the BTA-2 without the fibrils. 6. The standard amyloid fluorescent marker is thioflavin T (ThT): 20 μL of 0.8 mg/mL (2.51 mM) ThT in CH3CN and 100 μL of stock fibril solutions is diluted with 880 μL of pH 2 water.7. The emission spectra of BTA-2 in pH 2 water excited at 430 nm and excited at 360 nm, respectively. |
| References |
| Density | 1.188g/cm3 |
|---|---|
| Boiling Point | 428.8ºC at 760mmHg |
| Melting Point | 204-206ºC(lit.) |
| Molecular Formula | C16H16N2S |
| Molecular Weight | 268.37700 |
| Flash Point | 213.1ºC |
| Exact Mass | 268.10300 |
| PSA | 44.37000 |
| LogP | 4.33770 |
| Vapour Pressure | 1.48E-07mmHg at 25°C |
| Index of Refraction | 1.669 |
| Hazard Codes | Xi: Irritant; |
|---|---|
| Risk Phrases | 36/37/38 |
| Safety Phrases | 26-37/39 |
| HS Code | 2934999090 |
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10205-62-6 |
| Literature: Garmaise; Paris; Komlossy; Chambers; McCrae Journal of medicinal chemistry, 1969 , vol. 12, # 1 p. 30 - 36 |
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10205-62-6 |
| Literature: Garmaise; Paris; Komlossy; Chambers; McCrae Journal of medicinal chemistry, 1969 , vol. 12, # 1 p. 30 - 36 |
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10205-62-6 |
| Literature: Green Journal of the Chemical Society, 1889 , vol. 55, p. 228 Chemische Berichte, 1889 , vol. 22, p. 969 Full Text Show Details Cassella and Co. Patent: DE51738 ; Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 2, p. 299 |
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~88%
10205-62-6 |
| Literature: Yoon, Soon Byung; Chun, Eun Jeong; Noh, Young Ri; Yoon, Yong Jin; Lee, Sang-Geyong Bulletin of the Korean Chemical Society, 2013 , vol. 34, # 9 p. 2819 - 2821 |
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10205-62-6 |
| Literature: Green Journal of the Chemical Society, 1889 , vol. 55, p. 228 Chemische Berichte, 1889 , vol. 22, p. 969 Full Text Show Details Cassella and Co. Patent: DE51738 ; Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 2, p. 299 |
| HS Code | 2934999090 |
|---|---|
| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |