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369-07-3 864933-71-1

369-07-3

369-07-3 structure
369-07-3 structure
  • Name: ONPG
  • Chemical Name: 2-Nitrophenyl-beta-D-galactopyranoside
  • CAS Number: 369-07-3
  • Molecular Formula: C12H15NO8
  • Molecular Weight: 301.249
  • Catalog: Biochemical Carbohydrate Monosaccharide
  • Create Date: 2018-10-13 09:07:19
  • Modify Date: 2024-01-02 18:41:47
  • ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
Name 2-Nitrophenyl-beta-D-galactopyranoside
Synonyms EINECS 206-716-1
2-Nitrophenyl β-D-galactopyranoside
T6OTJ BOR BNW& CQ DQ EQ F1Q &&β-D-Galacto Form
MFCD00063255
2-Nitrophenyl β-D-Galactopyranoside [Substrate
o-Nitrophenyl β-D-galactopyranoside
β-D-Galactopyranoside, 2-nitrophenyl
2-Nitrophenyl β-D-galactoside
2-Nitrophenyl beta-D-Galactopyranoside
2-Nitrophenyl β-D-galactopyranoside (ONPG)
2-Nitrophenyl-β-D-galactopyranoside
ortho-Nitrophenyl-β-galactoside
ONPG
Description ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
Related Catalog
In Vitro The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1].
Kinase Assay The β-galactosidase activity is measured using two substrates including ONPG and lactose in this study. The β-galactosidase activity for ONPG is measured by following the amount o-nitrophenol released from ONPG. The reaction mixture is composed of 100 μL of the enzyme solution and 400 μL of ONPG solution (2.5 g/L in 100 mM Tris-HCl buffer at pH 6.8). After incubation at 78°C for 15 min, the reaction is terminated by adding an equal volume of 1 M Na2CO3. The released o-nitrophenol is quantitatively determined by measuring at A405. One unit of activity is defined as the amount of enzyme needed to produce 1 μmol of o-nitrophenol per minute under the assay condition. The specific activity is expressed as units per milligram of protein. Assays for activity towards lactose are performed in the same buffer containing 100 μL of enzyme solution and 5% lactose, and the reaction is stopped by boiling for 10 min, and the concentration of glucose is determined using a glucose oxidase-peroxidase assay kit. The released glucose is quantitatively determined by measuring A492. One unit of enzyme activity is defined as the amount of activity required to release 1 μmol of glucose per minute[1].
References

[1]. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237. doi: 10.1186/1471-2180-13-237.
Density 1.6±0.1 g/cm3
Boiling Point 572.4±50.0 °C at 760 mmHg
Melting Point 186-193 °C (dec.)
Molecular Formula C12H15NO8
Molecular Weight 301.249
Flash Point 300.0±30.1 °C
Exact Mass 301.079773
PSA 145.20000
LogP -0.78
Vapour Pressure 0.0±1.7 mmHg at 25°C
Index of Refraction 1.648
Storage condition 2~8°C
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi
Risk Phrases R36/37/38
Safety Phrases S22-S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 29400090
Precursor  0

DownStream  1

HS Code 29400090

Chemsrc provides CAS#:369-07-3 MSDS, density, melting point, boiling point, structure, formula, molecular weight, synthetic route, etc.
title: CAS No. 369-07-3 | Chemsrc address: https://www.chemsrc.com/en/baike/170639.html

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