GL Biochem (Shanghai) Ltd.
China
Product Name: Corticotropin Releasing Factor
Price: $Inquiry/1g
Purity: 98.0%
Stocking Period: Inquiry
Contact: Jackie Yang

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: CRF (bovine) trifluoroacetate salt
Price: ￥1058.0/1mg ￥2598.0/5mg
Purity: 90.0%
Stocking Period: 1 Day
Contact: Liu jia

Taiwan Oyi peptide Co. Ltd
China
Product Name: CRF (bovine) trifluoroacetate salt
Price: $Inquiry/1mg $Inquiry/100kg
Purity: 98.0%
Stocking Period: 1 Day
Contact: Vinnie

92307-52-3

92307-52-3 structure

92307-52-3 structure

Name: CRF (bovine) trifluoroacetate salt
Chemical Name: Corticotropin Releasing Factor, bovine
CAS Number: 92307-52-3
Molecular Formula: C₂₀₆H₃₄₀N₆₀O₆₃S
Molecular Weight: 4697.34
Catalog: Biochemical Peptide
Create Date: 2018-05-21 08:00:00
Modify Date: 2024-01-02 15:30:25
CRF,bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM.

Name	Corticotropin Releasing Factor, bovine
Synonyms	CRF,bovine

Description	CRF,bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM.
Related Catalog	Signaling Pathways >> GPCR/G Protein >> CRFR Peptides Research Areas >> Inflammation/Immunology
Target	Ki: 3.52 nM (CRF receptor)[1]
In Vitro	CRF,bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM[1]. CRF shows pEC50s of 11.16, 8.53 and 8.70 for human CRF1, human CRF2 and rat CRF2α[2]. CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC[3].
Cell Assay	CRF (1 μM) is added to the cell cultures that are further incubated for 5, 15 and 30 min at 37°C. Control cells are incubated with medium only. Following incubation time, cells are lysed directly on the growth dish using the detergent provided by the cAMP enzyme immunoassay kit. Following trypan blue staining to ensure complete lysis, the cell lysate is collected and assayed for cAMP. In some cases, the brain microvessel endothelial cells (BMEC) are treated with forskolin or pretreated either with the CRFR antagonist Antalarmin (1 μM) or the ATP analogue 2′5′-deoxyadenosine for 5 min at 37°C[3].
References	[1]. Hogg JE, et al. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. [2]. Smart D, et al. Characterisation using microphysiometry of CRF receptor pharmacology. Eur J Pharmacol. 1999 Aug 27;379(2-3):229-35. [3]. Esposito P, et al. Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells. Brain Res. 2003 Apr 11;968(2):192-8.

Molecular Formula	C₂₀₆H₃₄₀N₆₀O₆₃S
Molecular Weight	4697.34
Storage condition	−20°C

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