Native Pseudomonas sp. Creatinine amidohydrolase

Names

[ Name ]:
Native Pseudomonas sp. Creatinine amidohydrolase

[Synonym ]:
Creatinine amidohydrolase
Ethanone, 2,2,2-trifluoro-1-(4-methylphenyl)-
2,2,2-Trifluoro-1-p-tolyl-ethanone
2,2,2-Trifluoro-1-(4-methylphenyl)ethanone
Creatinine amide hydrolase
Native Pseudomonas sp. Creatinine amidohydrolase

Biological Activity

[Description]:

Creatininase (Creatinine amidohydrolase; CAH), namely creatinine amidohydrolase, from Pseudomonas putida, is a homohexameric enzyme commonly used in biochemical research. Creatininase acts on carbon-nitrogen bonds other than peptide bonds, and can catalyze the hydrolysis of creatinine to creatine, which can then be metabolized by creatinase to urea and sarcosine[1].

[Related Catalog]:

Research Areas >> Metabolic Disease

[References]

[1]. Barbara Beuth, et al. Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution. J Mol Biol. 2003 Sep 5;332(1):287-301.

Chemical & Physical Properties

[ Density]:
1.2±0.1 g/cm3

[ Boiling Point ]:
194.5±35.0 °C at 760 mmHg

[ Molecular Formula ]:
C₉H₇F₃O

[ Molecular Weight ]:
188.146

[ Flash Point ]:
78.1±17.4 °C

[ Exact Mass ]:
188.044907

[ LogP ]:
2.61

[ Appearance of Characters ]:
lyophilized powder

[ Vapour Pressure ]:
0.4±0.4 mmHg at 25°C

[ Index of Refraction ]:
1.453

[ Storage condition ]:
2-8°C

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)

[ Safety Phrases ]:
22-24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Analytical expression of non-steady-state concentrations and current pertaining to compounds present in the enzyme membrane of biosensor.

J. Phys. Chem. A 115(17) , 4299-306, (2011)

A mathematical model of trienzyme biosensor at an internal diffusion limitation for a non-steady-state condition has been developed. The model is based on diffusion equations containing a linear term ...

Immobilization of creatininase, creatinase and sarcosine oxidase on iron oxide nanoparticles/chitosan-g-polyaniline modified Pt electrode for detection of creatinine.

Enzyme Microb. Technol. 50(4-5) , 247-54, (2012)

Commercial enzymes, creatininase (CA) from Pseudomonas sp, creatinase (CI) from Pseudomonas sp, sarcosine oxidase (SO) from Bacillus sp were co-immobilized onto iron oxide nanoparticles/chitosan-graft...

Amperometric creatinine biosensor based on covalently coimmobilized enzymes onto carboxylated multiwalled carbon nanotubes/polyaniline composite film.

Anal. Biochem. 419(2) , 277-83, (2011)

A mixture of commercial creatinine amidohydrolase (CA), creatine amidinohydrolase (CI), and sarcosine oxidase (SO) was coimmobilized covalently via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC...