BODIPY FL Thapsigargin

BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells[1][2].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). BODIPY FL Thapsigargin[1]: 1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h. 2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 µM BODIPY FL TG for 5 min. 3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

[1]. Pérez-Gordones MC, et, al. Presence of a thapsigargin-sensitive calcium pump in Trypanosoma evansi: Immunological, physiological, molecular and structural evidences. Exp Parasitol. 2015 Dec;159:107-17.

[2]. Urbina DC, et, al. The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana. Biol Res. 2006;39(2):289-96.