Isopropyl unoprostone

Isopropyl unoprostone (Unoprostone isopropyl ester), an analogue of a prostaglandin metabolite, is a potent large conductance Ca2+-activated K+ (BK) channels activator. Isopropyl unoprostone has antiglaucoma effects, lowering intraocular pressure (IOP) by increasing aqueous humour outflow. Isopropyl unoprostone can improve retinal sensitivity and the protection of central retinal sensitivity[1][2].

Research Areas >> Others

Signaling Pathways >> Membrane Transporter/Ion Channel >> Potassium Channel

BK channels[1]

Isopropyl unoprostone (0.01-1 μM; 24 h) protects against H2O2-induced 661W cell death[2]. Isopropyl unoprostone (0.1-3 μM; 24 h) reduced the morphological change of 661W cells and protects against light-induced cell death[2]. Isopropyl unoprostone (0.001-1 μM; 48 h) suppresses light-induced phagocytotic dysfunction in ARPE-19 cells[2]. Apoptosis Analysis[2] Cell Line: 661W (treated with Isopropyl unoprostone for 1 h, then added H2O2) Concentration: 0.01, 0.1 and 1 μM Incubation Time: 24 h Result: Protected against H2O2-induced cell death in a concentration-dependent manner. Apoptosis Analysis[2] Cell Line: 661W (treated with Isopropyl unoprostone for 1 h, then exposed to 2,500 lux of white fluorescent light) Concentration: 0.1, 1 and 3 μM Incubation Time: 24 h Result: Protected against light-induced cell death in a concentration-dependent manner, and the effect was significant at the 1 and 3 μM concentrations; reduced the morphological change (the transition to a rounder shape) which is potentially indicative of a pre-apoptotic stage.

[1]. Haria M, et al. Unoprostone (isopropyl unoprostone). Drugs Aging. 1996 Sep;9(3):213-8; discussion 219-20. 

[2]. Tsuruma K, et al. Unoprostone reduces oxidative stress- and light-induced retinal cell death, and phagocytotic dysfunction, by activating BK channels. Mol Vis. 2011;17:3556-65.