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中性红

中性红用途

中性红是一种含氮pH指示剂,pKi为6.8,是类囊体内部酸化的指示剂。中性红将溶酶体染成红色[1]。

中性红名称

[ CAS 号 ]:
553-24-2

[ 中文名 ]:
中性红

[ 英文名 ]:
Neutral Red

[中文别名 ]:

[英文别名 ]:

Nuclear Fast Red
NEUTRAL RED
Neutral Red W
Toluylene Red
Neutral Red chloride
Michrome No. 226
Neutral Red (Tautomer)
Basic Red 5
EINECS 209-035-8
Neutral Red Base

中性红生物活性

[ 描述 ]:

中性红是一种含氮pH指示剂,pKi为6.8,是类囊体内部酸化的指示剂。中性红将溶酶体染成红色[1]。

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

随着细胞开始死亡,它们结合中性红细胞的能力减弱。中性红也用于对细胞培养物进行染色,以便对病毒进行平板滴定[2]。

[参考文献]

[1]. Winfried AUSLANDER, et al. NEUTRAL RED, A RAPID INDICATOR FOR pH-CHANGES IN THE INNER PHASE OF THYLAKOIDS. FEBS LETTERS. 1975 Nov.

[2]. Ellen Borenfreund, et al. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). Journal of tissue culture methods volume 9, pages7–9 (1985).

中性红物理化学性质

[ 沸点 ]:
497.3ºC at 760mmHg

[ 熔点 ]:
290 °C (dec.)(lit.)

[ 分子式 ]:
C₁₅H₁₇ClN₄

[ 分子量 ]:
288.775

[ 精确质量 ]:
288.114166

[ PSA ]:
55.04000

[ LogP ]:
4.12280

[ 外观性状 ]:
暗绿色粉末

[ 储存条件 ]:
库房通风低温干燥

[ 水溶解性 ]:
50 g/L

中性红MSDS

详细MSDS(安全技术说明书)

中性红毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1400000

CHEMICAL NAME :: Phenazine, 3-amino-7-(dimethylamino)-2-methyl-, hydrochloride

CAS REGISTRY NUMBER :: 553-24-2

LAST UPDATED :: 199701

DATA ITEMS CITED :: 22

MOLECULAR FORMULA :: C15-H16-N4.Cl-H

MOLECULAR WEIGHT :: 288.81

WISWESSER LINE NOTATION :: T C666 BN INJ E1 FZ LN1&1 &GH

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 112 mg/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 432 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 142 mg/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 96600 ug/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: Sex chromosome loss and nondisjunction

TYPE OF TEST :: Sister chromatid exchange

TYPE OF TEST :: DNA damage

TYPE OF TEST :: Cytogenetic analysis

MUTATION DATA

TEST SYSTEM :: Bird - chicken

DOSE/DURATION :: 85 ug/kg

REFERENCE :: 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1598 No. of Facilities: 420 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 694 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1598 No. of Facilities: 746 (estimated) No. of Industries: 13 No. of Occupations: 12 No. of Employees: 23740 (estimated) No. of Female Employees: 19202 (estimated)

中性红安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn:Harmful;

[ 风险声明 (欧洲) ]:
R68

[ 安全声明 (欧洲) ]:
S36/37

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
SG1400000

[ 海关编码 ]:
32041300

中性红制备

1.由N,N-二甲基对亚硝基苯胺盐酸盐与甲苯-2,4-二胺制取。

2.将二甲基苯胺加到5℃的盐酸水溶液中，搅拌使之溶解，然后边搅拌边加入亚硝酸钠水溶液进行反应，温度控制在5～10℃：

搅拌反应1H后，过滤，结晶对亚硝基二甲基苯胺盐酸盐用少量水洗涤后备用。将硫酸亚铁用90℃热水溶解，然后加入2，4－二氨基甲苯，充分搅拌溶解后，慢慢加入制得的对亚硝基二甲基苯胺盐酸盐，搅拌反应：

1h后，补加适量盐酸，过滤。滤液中通入氯化氢气体，静置，待结晶完全后过滤，干燥，即为中性红成品。

中性红海关

[ 海关编码 ]: 2933990090

[ 中文概述 ]:
2933990090. 其他仅含氮杂原子的杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途, 乌洛托品请注明外观, 6－己内酰胺请注明外观, 签约日期

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

中性红文献

Colon carcinogenesis is inhibited by the TRPM8 antagonist cannabigerol, a Cannabis-derived non-psychotropic cannabinoid.

Carcinogenesis 35(12) , 2787-97, (2014)

Cannabigerol (CBG) is a safe non-psychotropic Cannabis-derived cannabinoid (CB), which interacts with specific targets involved in carcinogenesis. Specifically, CBG potently blocks transient receptor ...

Particle pollution in Rio de Janeiro, Brazil: increase and decrease of pro-inflammatory cytokines IL-6 and IL-8 in human lung cells.

Environ. Pollut. 194 , 112-20, (2014)

Particle pollution from urban and industrialized regions in Rio de Janeiro (RJ), Brazil was analyzed for toxic and pro-inflammatory (cytokines: IL-6, IL-8, IL-10) responses in human bronchial epitheli...

Phenolics from Caesalpinia ferrea Mart.: antioxidant, cytotoxic and hypolipidemic activity.

Pharmazie 70 , 553-8, (2015)

Nine phenolics were isolated from the aqueous ethanol extract of the leaves of Caesalpinia ferrea. The isolates were characterized for the first time from that plant. The structures of all isolates (1...

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中性红