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结晶紫

结晶紫用途

结晶紫是革兰氏染色染料。

结晶紫名称

[ CAS 号 ]:
548-62-9

[ 中文名 ]:
结晶紫

[ 英文名 ]:
Crystal Violet

[中文别名 ]:

[英文别名 ]:

Crystal violet
Basic Violet 3
EINECS 208-953-6
Methyl violet
MFCD00011750

结晶紫生物活性

[ 描述 ]:

结晶紫是革兰氏染色染料。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[参考文献]

[1]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95.

[2]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C.

[3]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.

[相关活性小分子]

结晶紫物理化学性质

[ 密度 ]:
1.19 g/cm3 (20ºC)

[ 熔点 ]:
215ºC

[ 分子式 ]:
C₂₅H₃₀ClN₃

[ 分子量 ]:
407.979

[ 闪点 ]:
40ºC

[ 精确质量 ]:
407.212830

[ PSA ]:
9.49000

[ LogP ]:
1.46360

[ 外观性状 ]:
暗绿色粉末或晶体

[ 储存条件 ]:
库房通风低温干燥,与食品原料分开存放

结晶紫MSDS

结晶紫毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: BO9000000

CHEMICAL NAME :: Ammonium, (4-(bis(p-(dimethylamino)phenyl)methylene)-2,5-cycloh exadien-1-ylidene) dimethyl-, chloride

CAS REGISTRY NUMBER :: 548-62-9

LAST UPDATED :: 199710

DATA ITEMS CITED :: 48

MOLECULAR FORMULA :: C25-H30-N3.Cl

MOLECULAR WEIGHT :: 408.03

WISWESSER LINE NOTATION :: L6Y DYJ AUYR DN1&1&R DN1&1& DUK1&1 &Q &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Human

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Human

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Rodent - guinea pig

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 420 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 8900 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 96 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 5100 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: 1 gm/kg

TOXIC EFFECTS :: Behavioral - food intake (animal) Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - cat

DOSE/DURATION :: 100 mg/kg

TOXIC EFFECTS :: Gastrointestinal - hypermotility, diarrhea Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 150 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 5 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraduodenal

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 160 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 100 mg/kg

TOXIC EFFECTS :: Behavioral - ataxia

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 10 mg/kg

TOXIC EFFECTS :: Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 4 mg/kg/2D-I

TOXIC EFFECTS :: Gastrointestinal - peritonitis Related to Chronic Data - death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 10950 mg/kg/2Y-C

TOXIC EFFECTS :: Tumorigenic - Carcinogenic by RTECS criteria Endocrine - thyroid tumors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 25750 mg/kg/2Y-C

TOXIC EFFECTS :: Tumorigenic - Carcinogenic by RTECS criteria Kidney, Ureter, Bladder - tumors Reproductive - Tumorigenic effects - ovarian tumors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 100 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - musculoskeletal system Reproductive - Specific Developmental Abnormalities - urogenital system

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 40 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 100 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - other developmental abnormalities

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 7 mg/kg

SEX/DURATION :: female 6-19 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 7 mg/kg

SEX/DURATION :: female 6-19 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Non-mammalian species Cells - not otherwise specified

DOSE/DURATION :: 500 ug/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1517 No. of Facilities: 9858 (estimated) No. of Industries: 58 No. of Occupations: 52 No. of Employees: 79824 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1517 No. of Facilities: 3280 (estimated) No. of Industries: 21 No. of Occupations: 34 No. of Employees: 75632 (estimated) No. of Female Employees: 49319 (estimated)

结晶紫安全信息

[ 符号 ]:

GHS02, GHS07, GHS08, GHS09

[ 信号词 ]:
Warning

[ 危害声明 ]:
H226-H319-H351-H411

[ 警示性声明 ]:
P273-P281-P305 + P351 + P338

[ 个人防护装备 ]:
Eyeshields;Faceshields;full-face respirator (US);Gloves;multi-purpose combination respirator cartridge (US);type ABEK (EN14387) respirator filter

[ 危害码 (欧洲) ]:
T:Toxic

[ 风险声明 (欧洲) ]:
R22;R41;R45;R50/53

[ 安全声明 (欧洲) ]:
S45-S53-S60-S61

[ 危险品运输编码 ]:
UN 3077

[ WGK德国 ]:
3

[ RTECS号 ]:
BO9000000

[ 包装等级 ]:
III

[ 危险类别 ]:
9

[ 海关编码 ]:
32041300

结晶紫合成路线

结晶紫上下游产品

结晶紫上游产品

结晶紫下游产品

结晶紫制备

1.以 N，N-二甲基苯胺为原料，在硫酸铜、苯酚和食盐存在下经氧化、缩合而得，然后经碱性转化、酸性转化、盐析等精制步骤得精品，经过滤、干燥得成品。。。碱性紫 5BN 也可以通过米氏酮的路线合成，即：。
在反应器中加入 76mL 水、8g 无水硫酸铜、2.4g 苯酚、11.7g 食盐和添加剂(添加剂为表面活性剂或乳化剂。氧化缩合反应最佳投料比 (物质的量比) 为 N，N-二甲基苯胺:硫酸铜:苯酚:氯酸钠:添加剂=1:0.25:0.125:0.3:0.07)溶液 (5%) 35mL 和 24.2g N，N-二甲基苯胺，升温至 60℃，保温 3h 后，继续升温至 70℃，缓慢滴加 100mL 氯酸钠溶液 (含 NaClO3 6.4g)，于 4h 内加完，继续反应 1.5h。冷却，静置，倾去上层清液，下层固体即为氧化缩合产物，研细备用。
于反应器中加入 300mL 水、20.5mL 氢氧化钠溶液 (30%)，搅拌下加入上述氧化缩合产物。缓慢升温至 40℃，随后于 3h 内均匀升温至 80℃，再于 1h 内升温至 90℃，于 95℃保温 0.5h。然后补加水至总体积为 800mL，在 60-70℃滴加硫化钠溶液 (10%)，继续反应 1.5h。冷却，过滤，洗至 pH=8-9，滤饼备用。
于反应器中加 50mL 水、3mL 硫酸 (65%)，搅拌下加入上述滤饼，升温至 90-100℃保持 40min，趁热过滤，热水洗滤饼。合并滤液和洗液，60℃蒸发浓缩至约 50mL，冷却，盐析，静置，倾去清液，固体经干燥得染料产品。

2.制法在三氯化磷存在下，二甲基苯胺与p,p'- 二（二甲胺基）二苯基酮进行缩合反应，然后加酸溶解提取出结晶紫，并可用水进行重结晶精制。

结晶紫海关

[ 海关编码 ]: 32041300

结晶紫文献

Single nucleotide polymorphism analysis of the major tripartite multidrug efflux pump of Escherichia coli: functional conservation in disparate animal reservoirs despite exposure to antimicrobial chemotherapy.

Antimicrob. Agents Chemother. 54 , 1007-15, (2010)

AcrAB-TolC imparts a strong intrinsic resistance phenotype to many clinically significant molecules in Escherichia coli. This complex is composed of a pump, AcrB, and a periplasmic protein, AcrA, that...

Biologic activities of the TolC-like protein of Neisseria meningitidis as assessed by functional complementation in Escherichia coli.

Antimicrob. Agents Chemother. 54 , 506-8, (2010)

Neisseria meningitidis can produce a TolC-like protein needed for secretion of FrpC but not efflux of antimicrobials. We now report that expression of the meningococcal tolC gene in a TolC-deficient s...

Characterization of TolC efflux pump proteins from Pasteurella multocida.

Antimicrob. Agents Chemother. 52 , 4166-71, (2008)

Two TolC homologs, PM0527 and PM1980, were identified for Pasteurella multocida. A pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine ...

更多文献

结晶紫相关知识

龙胆紫的用途和制备

2022-06-01 20:35:37

龙胆紫，又名结晶紫，一般是暗绿色粉末或者结晶，其CAS号为548-62-9，化学结构式C25H30ClN3，闪点40摄氏度，龙胆紫还有一个俗名叫紫药水。龙胆紫溶于乙醇、氯仿,微溶于水、乙醚【1】。　　龙胆紫最早是用于化学染料，通常用于纸张、皮革、羽毛等染色，但是现在龙胆紫在生物医药上的染料用途越来...

结晶紫