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CldU(5-氯-2'-脱氧尿苷)

CldU(5-氯-2'-脱氧尿苷)用途

5-Chloro-2'-deoxyuridine,一个胸腺嘧啶的类似物,可用于研究次氯酸对 DNA 和 DNA 前体的破坏作用。

CldU(5-氯-2'-脱氧尿苷)名称

[ CAS 号 ]:
50-90-8

[ 中文名 ]:
5-氯-2-脱氧尿嘧啶核苷

[ 英文名 ]:
5-Chloro-2'-deoxyuridine

[英文别名 ]:

5-CHLORODEOXYURIDINE
MFCD00006531
5-CHLORODESOXYURIDINE
5-Chloro-2'-deoxy-D-uridine
CLUDR
5-Chlorodeoxyuridin
CldU
2'-DEOXY-5-CHLOROURIDINE
Chlorodeoxyuridine

CldU(5-氯-2'-脱氧尿苷)生物活性

[ 描述 ]:

5-Chloro-2'-deoxyuridine,一个胸腺嘧啶的类似物,可用于研究次氯酸对 DNA 和 DNA 前体的破坏作用。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 神经疾病

[体外研究]

当将5-氯-2'-脱氧尿苷(ClDU)置于组织培养基中时,哺乳动物细胞将类似物掺入DNA中。观察到培养基中10μM浓度的5-氯-2'-脱氧尿苷不改变细胞分裂动力学。以前已经表明,5-氯-2'-脱氧尿苷被代谢并使用选择性结合含有卤化碱基的DNA的抗体掺入DNA中。在这里报道的研究中,5-氯-2'-脱氧尿苷在作为T类似物时与BrdU更相似。 5-氯-2'-脱氧尿苷的毒性可部分归因于胸苷酸合成酶的抑制[1]。

[细胞实验]

在整个研究中使用人红白血病K-562细胞。首先以1.0×10 5个细胞/ mL接种细胞，并用10μM5-氯-2'-脱氧尿苷或10μM胸苷（阴性对照）处理63小时，或两次细胞倍增。通过台盼蓝排除计数细胞，通过离心沉淀（500×g）并用10mL无菌PBS [1]洗涤。

[动物实验]

大鼠[2]使用体重200-250g的成年雄性Wistar大鼠。首先，允许动物以0.05mg / kg /注射剂量自行给予甲基苯丙胺，以固定比例1（FR1）为基线时段。之后，将大鼠分成四组。两组大鼠（长期进入; LgA-4天（d），LgA-13d;每组n = 7）允许自身给予0.05mg / kg /注射甲基苯丙胺每天6小时，而其他组（短途访问; ShA-4 d，ShA-13 d;每组n = 7）允许每天1小时。所有程序都在黑暗循环期间执行。在第5天，ShA-4d和LgA-4d大鼠接受一次注射50mg / kg 5-氯-2'-脱氧尿苷并存活30分钟。在第14天，ShA-13d和LgA-13d在2小时后接受一次注射50mg / kg的5-碘-2'-脱氧尿苷，然后注射50mg / kg的5-氯-2'-脱氧尿苷。在注射5-氯-2'-脱氧尿苷后，这些大鼠也存活30分钟。平行组的药物初始大鼠（n = 6）接受一次注射50mg / kg IdU，然后在2小时后接受50mg / kg 5-氯-2'-脱氧尿苷。这些大鼠在注射5-氯-2'-脱氧尿苷后也能存活30分钟[2]。

[参考文献]

[1]. Kim CH, et al. Polymerase incorporation and miscoding properties of 5-chlorouracil. Chem Res Toxicol. 2010 Apr 19;23(4):740-8.

[2]. Yuan CJ, et al. Extended access methamphetamine decreases immature neurons in the hippocampus which results from loss and altered development of neural progenitors without altered dynamics of the S-phase of the cell cycle. Pharmacol Biochem Behav. 2011 Nov;100(1):98-108.

[相关活性小分子]

CldU(5-氯-2'-脱氧尿苷)物理化学性质

[ 密度 ]:
1.67 g/cm3

[ 熔点 ]:
176-179ºC

[ 分子式 ]:
C₉H₁₁ClN₂O₅

[ 分子量 ]:
262.64700

[ 精确质量 ]:
262.03600

[ PSA ]:
104.55000

[ 折射率 ]:
1.644

[ 储存条件 ]:
库房低温通风干燥

CldU(5-氯-2'-脱氧尿苷)MSDS

详细MSDS(安全技术说明书)

CldU(5-氯-2'-脱氧尿苷)毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: YU7400000

CHEMICAL NAME :: Uridine, 5-chloro-2'-deoxy-

CAS REGISTRY NUMBER :: 50-90-8

LAST UPDATED :: 199709

DATA ITEMS CITED :: 7

MOLECULAR FORMULA :: C9-H11-Cl-N2-O5

MOLECULAR WEIGHT :: 262.67

WISWESSER LINE NOTATION :: T6NVMVJ EG A- ET5OTJ B1Q CQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 2 gm/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: Mutation in mammalian somatic cells

TEST SYSTEM :: Rodent - hamster Ovary

DOSE/DURATION :: 500 nmol/L

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 4,301,1982 *** REVIEWS *** TOXICOLOGY REVIEW 32XPAD "Teratology," Berry, C.L., and D.E. Poswillo, eds., New York, Springer, 1975 Volume(issue)/page/year: -,49,1975

CldU(5-氯-2'-脱氧尿苷)安全信息

[ 符号 ]:

GHS07, GHS08

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302-H312-H332-H351

[ 警示性声明 ]:
P280

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Gloves

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R20/21/22

[ 安全声明 (欧洲) ]:
36/37/39-45

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
YU7400000

[ 海关编码 ]:
2942000000

CldU(5-氯-2'-脱氧尿苷)合成路线

详细合成路线

CldU(5-氯-2'-脱氧尿苷)上下游产品

CldU(5-氯-2'-脱氧尿苷)上游产品

CldU(5-氯-2'-脱氧尿苷)下游产品

CldU(5-氯-2'-脱氧尿苷)海关

[ 海关编码 ]: 2942000000

CldU(5-氯-2'-脱氧尿苷)文献

RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.

Hum. Mol. Genet. 23(16) , 4232-48, (2014)

The MRE11/RAD50/NBN (MRN) complex plays a key role in detecting DNA double-strand breaks, recruiting and activating ataxia-telangiectasia mutated and in processing the breaks. Members of this complex ...

Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication.

Mol. Cell. Biol. 35(4) , 699-715, (2015)

DNA rereplication is a major form of aberrant replication that causes genomic instabilities, such as gene amplification. However, little is known about which DNA polymerases are involved in the proces...

Role of the Exocyst Complex Component Sec6/8 in Genomic Stability.

Mol. Cell. Biol. 35 , 3633-45, (2015)

The exocyst is a heterooctomeric complex well appreciated for its role in the dynamic assembly of specialized membrane domains. Accumulating evidence indicates that this macromolecular machine also se...

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