<生产厂家价格>

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)用途

CFSE 是可以跟踪细胞分裂的荧光染料。

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)名称

[ CAS 号 ]:
150347-59-4

[ 中文名 ]:
5(6)-羧基二乙酸荧光素琥珀酰亚胺酯

[ 英文名 ]:
5-Carboxyfluorescein diacetate N-succinimidyl ester

[英文别名 ]:

Carboxyfluorescein diacetate succinimidyl ester
5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate
MFCD00037466
5-CFDA N-succinimidyl ester
CFSE

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)生物活性

[ 描述 ]:

CFSE 是可以跟踪细胞分裂的荧光染料。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

CFSE是一种荧光染料,可以跟踪细胞分裂[1]。为了检查免疫细胞迁移到活切片中的能力,分离自体脾细胞或PBMC的单细胞悬浮液,用CFSE标记它们,并在培养的第2天将它们添加到切片的顶部。再培养6天后,发现CFSE标记的免疫细胞(绿色)已经在整个切片中迁移。没有添加CFSE标记的免疫细胞,切片中未发现阳性细胞[2]。

[细胞实验]

将脾细胞（或PBMC）的单细胞悬液用PBS中的1μMCFSE在37℃下染色9分钟，在室温下与20mL 10％FBS RPMI-1640培养基一起染色2分钟，离心，洗涤并计数。将2×106个CFSE标记的脾细胞加入到24孔膜培养插入物中的切片顶部[2]。

[参考文献]

[1]. Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005307.

[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

[相关活性小分子]

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)物理化学性质

[ 密度 ]:
1.6±0.1 g/cm3

[ 沸点 ]:
757.9±70.0 °C at 760 mmHg

[ 熔点 ]:
152-154ºC(lit.)

[ 分子式 ]:
C₂₉H₁₉NO₁₁

[ 分子量 ]:
557.461

[ 闪点 ]:
412.2±35.7 °C

[ 精确质量 ]:
557.095825

[ PSA ]:
151.81000

[ LogP ]:
0.50

[ 外观性状 ]:
无色或淡黄色固体

[ 蒸汽压 ]:
0.0±2.6 mmHg at 25°C

[ 折射率 ]:
1.701

[ 储存条件 ]:
-20°C

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi

[ 危险品运输编码 ]:
NONH for all modes of transport

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDA)文献

MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b.

J. Immunol. 194(5) , 2079-88, (2015)

Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC I...

Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone.

Tissue Eng. Part A 21(5-6) , 948-59, (2015)

Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bon...

Olanzapine inhibits proliferation, migration and anchorage-independent growth in human glioblastoma cell lines and enhances temozolomide's antiproliferative effect.

J. Neurooncol. 122(1) , 21-33, (2015)

The poor prognosis of patients with glioblastoma fuels the search for more effective therapeutic compounds. We previously hypothesised that the neuroleptic olanzapine may enhance antineoplastic effect...

更多文献