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5-溴-4-氯-3-吲哚基β-D-葡萄糖苷酸钠盐

用途

X-Gluc钠是检测β-葡萄糖醛酸酶(一种由大肠杆菌产生的酶)的染料试剂。X-谷氨酸钠可用于检测食品、水和尿路中的大肠杆菌污染。X-Gluc钠还广泛用于分子生物学实验,以标记和检测目标基因的表达(GUS报告系统)[1]。

名称

[ CAS 号 ]:
129541-41-9

[ 中文名 ]:
5-溴-4-氯-3-吲哚基-beta-D-葡糖苷酸钠

[ 英文名 ]:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt

[中文别名 ]:

[英文别名 ]:

BC-Indicator,X-GlcA,X-glucuronide
X-gluc/X-GlcA
X-Glu,CHA Salt
X-Gluc Sodium Salt
X-GlcA,X-glucuronide CHA salt
5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E
X-Gluc CHA Salt
X-GlcA Sodium Salt
X-glucuronide CHA salt
5-Bromo-4-chlo

生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

指南(以下是我们推荐的方案。该方案仅提供指南,应根据您的具体需要进行修改)。1.将20mg X-Gluc钠溶于1mL二甲基甲酰胺(DMF)中,制备X-Gluc-主混合物。2.将制备好的X-Gluc溶液以最终浓度50μg/mL添加到琼脂培养基平板中,无需灭菌。3.让平板风干,并用于接种微生物。4.在35°C下培养平板,接种后观察16-24小时。

[参考文献]

[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.

物理化学性质

[ 沸点 ]:
732.2ºC at 760mmHg

[ 熔点 ]:
228-230°C

[ 分子式 ]:
C₂₀H₂₆BrClN₂O₇

[ 分子量 ]:
521.787

[ 闪点 ]:
396.6ºC

[ 精确质量 ]:
520.061157

[ PSA ]:
135.07000

[ 外观性状 ]:
白色固体

[ 储存条件 ]:
−20°C

MSDS

详细MSDS(安全技术说明书)

安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi

[ 风险声明 (欧洲) ]:
R36/37/38

[ 安全声明 (欧洲) ]:
S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ 海关编码 ]:
29339990

文献

Automated Sampling Procedures Supported by High Persistence of Bacterial Fecal Indicators and Bacteroidetes Genetic Microbial Source Tracking Markers in Municipal Wastewater during Short-Term Storage at 5°C.

Appl. Environ. Microbiol. 81 , 5134-43, (2015)

Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate ...

Quality changes of fresh-cut pomegranate arils during shelf life as affected by deficit irrigation and postharvest vapour treatments.

J. Sci. Food Agric. 95 , 2325-36, (2015)

The effect of two sustained deficit irrigation (SDI) strategies, compared to a control, on postharvest physicochemical, microbial, sensory quality attributes and anthocyanin content of fresh-cut pomeg...

Comparison of Six Chromogenic Agar Media for the Isolation of a Broad Variety of Non-O157 Shigatoxin-Producing Escherichia coli (STEC) Serogroups.

Int. J. Environ. Res. Public Health 12 , 6965-78, (2015)

The isolation of non-O157 STEC from food samples has proved to be challenging. The selection of a suitable selective isolation agar remains problematic. The purpose of this study was to qualitatively ...

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