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ADHP

ADHP用途

ADHP 是一种荧光过氧化物酶底物(λex=530 nm, λem=590 nm)。

ADHP名称

[ CAS 号 ]:
119171-73-2

[ 中文名 ]:
10-乙酰基-3,7-二羟基吩嗪

[ 英文名 ]:
Ampliflu Red

[中文别名 ]:

10-乙酰基-3,7-二羟基苯噁嗪

[英文别名 ]:

10-Acetyl-10H-phenoxazine-3,7-diol
1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone
1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
MFCD00467861
N-acetyl-3,7-dihydroxyphenoxazine
10H-Phenoxazine-3,7-diol, 10-acetyl-
Ampliflu™ Red
Amplex red
Ethanone, 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-
10-Acetyl-3,7-dihydroxyphenoxazine

ADHP生物活性

[ 描述 ]:

ADHP 是一种荧光过氧化物酶底物(λex=530 nm, λem=590 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

为了获得化合物I的参数Km和kcat,使用两种独立的方法。最初,研究了使用内置于荧光板读取器的注射器功能来氧化ADHP。自动注射器分配H2O2以引发反应,作为产生一组进展曲线的手段。对MPO介导的ADHP氧化的分析得出Km为31±4μM,kcat为186±6s-1。在1~80μM的ADHP浓度的实验范围内,kobs也增加,并且对于保持基质的相反实验恒定的3至45 nM MPO。从kobs对ADHP浓度Kappon的斜率获得的表观二阶速率常数是2.1±0.2mM/s [1]。

[激酶实验]

将ADHP，4-ABAH，2-ABAH，4-BAH，4-FBAH，4-NBAH，4-TFMBAH，3-DMABAH，NaN 3和异烟肼溶解在DMSO中，随后稀释到测定缓冲液中。反应中DMSO的最终浓度小于0.5％（v / v），这不影响氧化的ADHP产物7-羟基-3H-吩恶嗪-3-酮（试卤灵）的荧光。将ADHP（20μM）的反应与MPO（2.8nm）在测定缓冲液中孵育，并通过从连续稀释池中加入1/10体积的H 2 O 2引发。为了确定最简单的苯甲酸酰肼抑制剂或其类似物4-TFMBAH对MPO的血红素催化能力的影响，将MPO（1.2μM）与不同浓度的BAH抑制剂（0,0.025,0.25,2.5）一起孵育10分钟使用ADHP（40μM），12.5和25mM），并在加入H 2 O 2（20μM）ADHP后测量反应时间。在室温下在测定缓冲液中测量所有反应。将20μL样品加入非还原性样品上样缓冲液中，然后在不事先加热的情况下加载，并通过4-15％梯度SDS-聚丙烯酰胺凝胶电泳分离[1]。

[参考文献]

[1]. Jiansheng Huang, et al. Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs. Arch Biochem Biophys. 2014 Apr 15; 548: 74–85.

[相关活性小分子]

ADHP物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 沸点 ]:
618.6±55.0 °C at 760 mmHg

[ 分子式 ]:
C₁₄H₁₁NO₄

[ 分子量 ]:
257.241

[ 闪点 ]:
327.9±31.5 °C

[ 精确质量 ]:
257.068817

[ PSA ]:
70.00000

[ LogP ]:
0.89

[ 外观性状 ]:
白色至棕色至棕色的粉末或固体

[ 蒸汽压 ]:
0.0±1.9 mmHg at 25°C

[ 折射率 ]:
1.689

[ 储存条件 ]:
2~8℃，干燥，密封

ADHPMSDS

详细MSDS(安全技术说明书)

ADHP安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Gloves

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R22

[ 危险品运输编码 ]:
NONH for all modes of transport

[ 海关编码 ]:
2934999090

ADHP海关

[ 海关编码 ]: 2934999090

[ 中文概述 ]:
2934999090. 其他杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

ADHP文献

Human recombinant cytochrome P450 enzymes display distinct hydrogen peroxide generating activities during substrate independent NADPH oxidase reactions.

Toxicol. Sci. 141(2) , 344-52, (2014)

Microsomal enzymes generate H2O2 in the presence of NADPH. In this reaction, referred to as "oxidase" activity, H2O2 is generated directly or indirectly via the formation of superoxide anion. In the p...

Statin-induced inhibition of breast cancer proliferation and invasion involves attenuation of iron transport: intermediacy of nitric oxide and antioxidant defence mechanisms.

FEBS J. 281(16) , 3719-38, (2014)

Accumulating evidence from in vitro, in vivo, clinical and epidemiological studies shows promising results for the use of statins against many cancers including breast carcinoma. However, the molecula...

Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors.

Lab Chip 15 , 2162-72, (2015)

A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol-ene chemistry is presented. The method allows monolith synthesis and anchor...

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