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荧光钙探针 Fura 2-AM

荧光钙探针 Fura 2-AM用途

Fura-2 AM 是一种比率荧光 Ca2+ 指示剂。

荧光钙探针 Fura 2-AM名称

[ CAS 号 ]:
108964-32-5

[ 中文名 ]:
荧光钙探针 Fura 2-AM

[ 英文名 ]:
Fura-2, AM

[中文别名 ]:

[英文别名 ]:

Fura 2am
Fura-2 pentakis(acetoxymethyl) ester
MFCD00036976
Fura-2 AM

荧光钙探针 Fura 2-AM生物活性

[ 描述 ]:

Fura-2 AM 是一种比率荧光 Ca2+ 指示剂。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

在装载750nM(n = 111),500nM(n = 119)或250nM(n = 130)Fura-2AM的tsA-201细胞中设想胰蛋白酶(100nM)介导的Ca2 +瞬变,并比较平均值。 Ca2 +在基线以上增加至使用1μMFura-2AM在初始实验中获得的值。对于750,500和250 nM,Ca2 +增加到基线以上是633.0±33.9 nM(n = 111,P> 0.05与1μMFura-2 AM相比),552.6±22.7 nM(n = 119,P> 0.05比较至1μMFura-2 AM)和616.0±24.9 nM(n = 130,P> 0.05与1μMFura-2 AM相比),分别为[1]。

[细胞实验]

细胞负载Fura-2AM，其扩散穿过细胞膜并通过细胞酯酶去酯化以产生富含Fura-2的酸。将2mL培养基等分至15mL锥形管中，温热至37℃。加入2μLFura-2 AM原液，生成1μMFura-2 AM溶液。剧烈涡旋溶液1分钟。将加样溶液转移到35mm组织培养皿中，并将盖玻片与细胞一起转移到培养皿中。在黑暗的培养箱中将神经元在37度孵育30分钟。准备一个35毫米的培养皿，其中含有2毫升不含Fura-2 AM的组织培养基。从装载溶液中取出盖玻片并放入新盘中。将盖玻片安装在成像室[2]上。

[参考文献]

[1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24.

[2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067.

[相关活性小分子]

荧光钙探针 Fura 2-AM物理化学性质

[ 密度 ]:
1.4±0.1 g/cm3

[ 沸点 ]:
975.9±75.0 °C at 760 mmHg

[ 分子式 ]:
C₄₄H₄₇N₃O₂₄

[ 分子量 ]:
1001.850

[ 闪点 ]:
544.0±37.1 °C

[ 精确质量 ]:
1001.254944

[ PSA ]:
327.11000

[ LogP ]:
2.90

[ 蒸汽压 ]:
0.0±0.3 mmHg at 25°C

[ 折射率 ]:
1.567

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
DMSO: soluble

荧光钙探针 Fura 2-AMMSDS

详细MSDS(安全技术说明书)

荧光钙探针 Fura 2-AM安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

荧光钙探针 Fura 2-AM文献

Neuroprotection against degeneration of sk-N-mc cells using neuron growth factor-encapsulated liposomes with surface cereport and transferrin.

J. Pharm. Sci. 103(8) , 2484-97, (2014)

Liposomes with Cereport (RMP-7) and transferrin (Tf) (RMP-7/Tf/liposomes) were employed to target the blood-brain barrier (BBB) and to inhibit the degeneration of neurons insulted with fibrillar β-amy...

Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation.

Stem Cell Res. Ther. 6 , 83, (2015)

Induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) may be suitable for myocardial repair. While their functional and structural properties have been extensively investigated, their respons...

Hypobaric Preconditioning Modifies Group I mGluRs Signaling in Brain Cortex.

Neurochem. Res. 40 , 2200-10, (2015)

The study assessed involvement of Ca(2+) signaling mediated by the metabotropic glutamate receptors mGluR1/5 in brain tolerance induced by hypoxic preconditioning. Acute slices of rat piriform cortex ...

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