5-carboxyfluorescein

Modify Date: 2024-01-08 19:00:30

5-carboxyfluorescein Structure
5-carboxyfluorescein structure
Common Name 5-carboxyfluorescein
CAS Number 76823-03-5 Molecular Weight 376.316
Density 1.7±0.1 g/cm3 Boiling Point 725.2±60.0 °C at 760 mmHg
Molecular Formula C21H12O7 Melting Point 368-372°C
MSDS Chinese USA Flash Point 266.9±26.4 °C

 Use of 5-carboxyfluorescein


The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

 Names

Name 5-carboxyfluorescein
Synonym More Synonyms

 5-carboxyfluorescein Biological Activity

Description The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
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 Chemical & Physical Properties

Density 1.7±0.1 g/cm3
Boiling Point 725.2±60.0 °C at 760 mmHg
Melting Point 368-372°C
Molecular Formula C21H12O7
Molecular Weight 376.316
Flash Point 266.9±26.4 °C
Exact Mass 376.058289
PSA 113.29000
LogP 2.62
Vapour Pressure 0.0±2.5 mmHg at 25°C
Index of Refraction 1.816
Storage condition Store at 2-8
Water Solubility DMF: soluble

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi: Irritant;
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2932999099

 Synthetic Route

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5-carboxyfluorescein Structure

5-carboxyfluorescein

CAS#:76823-03-5

Literature: Russian Journal of General Chemistry, , vol. 65, # 3.2 p. 454 - 456 Zhurnal Obshchei Khimii, , vol. 65, # 3 p. 511 - 513

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5-carboxyfluorescein Structure

5-carboxyfluorescein

CAS#:76823-03-5

Literature: Chemical communications (Cambridge, England), , # 23 p. 2870 - 2871

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5-carboxyfluorescein Structure

5-carboxyfluorescein

CAS#:76823-03-5

Literature: Journal of the American Chemical Society, , vol. 135, # 47 p. 17659 - 17662

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5-carboxyfluorescein Structure

5-carboxyfluorescein

CAS#:76823-03-5

Literature: Chemische Berichte, , vol. 11, p. 1342

 Customs

HS Code 2932999099
Summary 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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 Synonyms

MFCD00151081
3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-dihydroxy-3-oxo-
EINECS 276-331-1
3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
5-carboxyfluorescein
5-FAM
Top Suppliers:I want be here


  • DC Chemicals Limited
  • China
  • Product Name: 5-FAM
  • Price: $Inquiry/5g $80.0/500mg $135.0/1g
  • Purity: 98.0%
  • Stocking Period: 3 Day
  • Contact: Tony Cao



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Price: $66/10mM*1mLinDMSO

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