Ethidium bromide structure
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Common Name | Ethidium bromide | ||
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CAS Number | 1239-45-8 | Molecular Weight | 394.308 | |
Density | N/A | Boiling Point | N/A | |
Molecular Formula | C21H20BrN3 | Melting Point | 260-262 °C (dec.)(lit.) | |
MSDS | Chinese USA | Flash Point | >100°C | |
Symbol |
GHS06, GHS08 |
Signal Word | Danger |
Use of Ethidium bromideEthidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. |
Name | ethidium bromide |
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Synonym | More Synonyms |
Description | Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. |
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Related Catalog | |
In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2. Mix the agarose powder with 1X TAE/TBE. 3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4. Make the solution cool down before solidification. 5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel). 6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection. |
References |
Melting Point | 260-262 °C (dec.)(lit.) |
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Molecular Formula | C21H20BrN3 |
Molecular Weight | 394.308 |
Flash Point | >100°C |
Exact Mass | 393.084045 |
PSA | 55.92000 |
LogP | 2.29820 |
Index of Refraction | 1.4575 (20ºC) |
Storage condition | 2-8°C |
Stability | Stable. Incompatible with strong oxidizing agents. |
Water Solubility | 40 g/L (25 ºC) |
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATAACUTE TOXICITY DATA
MUTATION DATA
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Symbol |
GHS06, GHS08 |
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Signal Word | Danger |
Hazard Statements | H302-H330-H341 |
Precautionary Statements | P260-P281-P284-P310 |
Personal Protective Equipment | Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges |
Hazard Codes | T+:Verytoxic; |
Risk Phrases | R21/22;R26;R36/37/38;R68 |
Safety Phrases | S36/37-S45-S36/37/39-S28A-S26-S22 |
RIDADR | UN 2811 6.1/PG 1 |
WGK Germany | 3 |
RTECS | SF7950000 |
Packaging Group | I |
Hazard Class | 6.1 |
HS Code | 29339990 |
Precursor 9 | |
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DownStream 1 | |
HS Code | 2933990090 |
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Summary | 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
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3,8-Diamino-5 |
MFCD00011724 |
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide |
Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1) |
Homidium bromide |
3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide |
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide |
EINECS 214-984-6 |
5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide |
EthidiuM BroMide |