DC Chemicals Limited
China
Product Name: T0070907
Price: $250.0/100mg $500.0/250mg $1000.0/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 2-CHLORO-5-NITRO-N-4-PYRIDINYLBENZAMIDE
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

313516-66-4

313516-66-4 structure

313516-66-4 structure

Name: T0070907
Chemical Name: 2-chloro-5-nitro-N-pyridin-4-ylbenzamide
CAS Number: 313516-66-4
Molecular Formula: C₁₂H₈ClN₃O₃
Molecular Weight: 277.663
Catalog: Biochemical Inhibitor DNA damage PPAR inhibitor
Create Date: 2019-01-03 10:15:07
Modify Date: 2024-01-09 19:04:57
T0070907 is a potent PPARγ antagonist with a Ki of 1 nM.

Name	2-chloro-5-nitro-N-pyridin-4-ylbenzamide
Synonyms	2-Chloro-5-nitro-N-4-pyridinyl-benzamide Benzamide,2-chloro-5-nitro-N-4-pyridinyl Benzamide,2-chloro-5-nitro-N-4-pyridinyl 2-chloro-5-nitro-N-pyridin-4-yl-benzamide Benzamide, 2-chloro-5-nitro-N-4-pyridinyl- 2-Chloro-5-nitro-N-(4-pyridinyl)benzamide 2-Chloro-5-nitro-N-4-pyridinylbenzamide 2-chloro-5-nitro-N-(pyridin-4-yl)benzamide T0070907

Description	T0070907 is a potent PPARγ antagonist with a Ki of 1 nM.
Related Catalog	Signaling Pathways >> Cell Cycle/DNA Damage >> PPAR Signaling Pathways >> Cell Cycle/DNA Damage >> RAD51 Research Areas >> Cancer
Target	PPARγ:1 nM (Ki) PPARδ:1.8 μM (Ki) PPARα:0.85 μM (Ki)
In Vitro	T0070907 (50 μM) pre-treatment impairs repair of IR-induced DNA DSBs in both ME-180 and SiHa cells treated with irradiated (4 Gy). T0070907 (0-50 μM) significantly decreases the levels of DNA-PKcs and RAD51 proteins in ME-180 and SiHa cells[1]. T0070907 (50 μM) treatment reduces the levels of α- and β-tubulin protein in a time-dependent manner, decreases the synthesis of DNA, and prevents the radiation-induced alterations in the cell cycle regulatory proteins of ME180 and SiHa cells[2]. T0070907 (10 µM) has cytotoxicity in an adipocyte-specific and PPARγ-independent manner. T0070907 increases oxidative stress in immature adipocytes[3]. T0070907 (1 μM) blocks the induction of adipogenesis by various treatments of the adipogenic cell line 3T3-L1. T0070907 covalently modifies PPAR on cysteine 313 in helix 3 of human PPAR 2[4].
Kinase Assay	To determine the binding affinity of T0070907 to the PPARs, scintillation proximity assay (SPA) is performed with the following modifications. A 90 μL reaction contains SPA buffer (10 mM K2HPO4, 10 mM KH2PO4, 2 mM EDTA, 50 mM NaCl, 1 mM dithiothreitol, 2 mM CHAPS, 10% (v/v) glycerol, pH 7.1), 50 ng of GST-PPAR (or 150 ng of GST-PPAR), 5 nM 3H-labeled radioligands, and 5 μL of T0070907 in Me2SO. After incubation for 1 h at room temperature, 10 μL of polylysine-coated SPA beads (at 20 mg/mL in SPA buffer) are added, and the mixture is incubated for 1 h before reading in Packard Topcount. [3H]Rosiglitazone is used for PPAR, and [3H]GW2433 is used for PPAR and PPAR.
References	[1]. An Z, et al. T0070907 inhibits repair of radiation-induced DNA damage by targeting RAD51. Toxicol In Vitro. 2016 Dec;37:1-8 [2]. An Z, et al. T0070907, a PPAR γ inhibitor, induced G2/M arrest enhances the effect of radiation in human cervical cancer cells through mitotic catastrophe. Reprod Sci. 2014 Nov;21(11):1352-61. [3]. Kawahara A, et al. Peroxisome proliferator-activated receptor γ (PPARγ)-independent specific cytotoxicity against immature adipocytes induced by PPARγ antagonist T0070907. Biol Pharm Bull. 2013;36(9):1428-34 [4]. Lee G, et al. T0070907, a selective ligand for peroxisome proliferator-activated receptor gamma, functions as an antagonist of biochemical and cellular activities. J Biol Chem. 2002 May 31;277(22):19649-57. Epub 2002 Mar 4

Density	1.5±0.1 g/cm3
Boiling Point	381.7±37.0 °C at 760 mmHg
Molecular Formula	C₁₂H₈ClN₃O₃
Molecular Weight	277.663
Flash Point	184.6±26.5 °C
Exact Mass	277.025421
PSA	87.81000
LogP	2.25
Appearance	white
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.684
Storage condition	Store at RT
Water Solubility	DMSO: >10mg/mL \| Soluble in DMSO at 10mg/ml. Insoluble in water.

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302-H319
Precautionary Statements	P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn
Risk Phrases	22-36
Safety Phrases	26
RIDADR	NONH for all modes of transport
HS Code	2933399090

504-24-5 structure

504-24-5

25784-91-2 structure

25784-91-2

~%

313516-66-4 structure

313516-66-4

Literature: US2003/134859 A1, ;

Precursor 2
504-24-5 25784-91-2
DownStream 0

HS Code	2933399090
Summary	2933399090. other compounds containing an unfused pyridine ring (whether or not hydrogenated) in the structure. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%