Name | 7,9-dihydro-1H-purine-2,6,8(3H)-trione |
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Synonyms |
2,6,8-trioxypurine
1H-Purine-2,6,8 (3H)-trione, 7,9-dihydro- 1H-Purine-2,6,8(3H)-trione, 7,9-dihydro- [14C]-Uric acid 2,6,8-trioxopurine EINECS 200-720-7 7,9-Dihydro-1H-purine-2,6,8(3H)-trione 1H-Purine-2,6,8-triol urate [3H]-Uric acid 2,6,8-trihydroxypurine Uric Acid Uric acid (8CI) MFCD00005712 1H-Purine-2,6,8(3H)-trione, 7,9-dihydro- (9CI) 1H-Purine-2,6,8(3H)-trione,7,9-dihydro Lithic acid 7,9-dihydro-3H-purine-2,6,8-trione |
Description | Uric acid is an endogenous antioxidant that scavenges reactive oxygen species (ROS) including singlet oxygen, oxygen radicals, and peroxynitrite. |
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Related Catalog | |
Target |
Human Endogenous Metabolite |
In Vitro | Uric acid is an endogenous antioxidant that scavenges reactive oxygen species (ROS) including singlet oxygen, oxygen radicals, and peroxynitrite. Incubation with indomethacin significantly increases malondialdehyde (MDA) levels in Caco-2 cells compare to those not treated indomethacin. Incubation with both indomethacin and Uric acid significantly decreases MDA levels compare to those grown in the presence of indomethacin alone. Co-treatment of cells with indomethacin and Uric acid significantly decreases ROS levels compare to those in cells incubated with indomethacin alone. Cell viability in Caco-2 cells treated with both indomethacin and Uric acid is higher than that in cells treated with indomethacin alone. Uric acid has a protective effect on indomethacin-induced intestinal cell changes through its antioxidant activity[1]. |
In Vivo | When mice treated with indomethacin are concurrently administered Uric acid orally, ulcer areas are significantly reduced, in a Uric acid dose-dependent manner. Indomethacin increases the ratio of crypt depth to villous height in the ileum, while the ratio is significantly lower when mice are concurrently administered Uric acid orally. Administration of indomethacin also increases the histopathological score of tissue damage in the small intestine, while mice concurrently administered Uric acid orally has a significantly lower histopathological score. The ileal levels of malondialdehyde (MDA) in indomethacin-induced enteropathy model mice orally administered Uric acid are also significantly lower than the levels in mice administered indomethacin alone[1]. |
Cell Assay | Human colon carcinoma cells (Caco-2) are used and grown in Eagle’s minimum essential medium supplemented with 10% FBS, 1% non-essential amino acid mixture, and 1% Pen-Strep at 37°C in a humidified atmosphere with 5% CO2. Caco-2 cells are incubated with indomethacin in the presence or absence of Uric acid for 24 or 48 hours[1]. |
Animal Admin | To evaluate the effect of oral administration of Uric acid on NSAID-induced enteropathy, mice are given Uric acid (2.5, 10, 25, 100, or 250 mg/kg body weight) suspended in 0.5% carboxymethyl cellulose orally at 30 minutes before, and 12 hours after indomethacin or vehicle treatment. To evaluate the effect of intraperitoneal administration of inosinic acid plus potassium oxonate on NSAID-induced enteropathy, mice are given inosinic acid (500 mg/kg body weight) plus potassium oxonate (250 mg/kg body weight) 24 intraperitoneally at 30 minutes before, and 12 hours after indomethacin or vehicle treatment[1]. |
References |
Density | 1.9±0.1 g/cm3 |
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Boiling Point | 863ºC at 760 mmHg |
Melting Point | >300 °C(lit.) |
Molecular Formula | C5H4N4O3 |
Molecular Weight | 168.110 |
Flash Point | 475.7ºC |
Exact Mass | 168.028336 |
PSA | 114.37000 |
LogP | -1.08 |
Index of Refraction | 1.721 |
Storage condition | Store at RT. |
Stability | Stable. Incompatible with acids, bases, oxidising agents. |
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATAACUTE TOXICITY DATA
MUTATION DATA
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Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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Hazard Codes | Xi |
Risk Phrases | R33 |
Safety Phrases | S24/25 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
RTECS | YU7050080 |
HS Code | 2933990090 |
Precursor 10 | |
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DownStream 10 | |
HS Code | 2933990090 |
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Summary | 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |