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Related CAS#:
477-73-6 81-93-6
2390-56-9 4569-88-4
7006-08-8

477-73-6

477-73-6 structure
477-73-6 structure
  • Name: Safranin
  • Chemical Name: 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine,chloride
  • CAS Number: 477-73-6
  • Molecular Formula: C20H19ClN4
  • Molecular Weight: 350.845
  • Catalog: Dyes and pigments dye Basic dye
  • Create Date: 2018-02-05 08:00:00
  • Modify Date: 2024-01-02 17:23:16
  • Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration[1].
Name 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine,chloride
Synonyms Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride (1:1)
Safranin T
safranin A
C.I. Basic Red 2 (8CI)
Safranine
safranin Y
3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chloride
Basic Red 2
Gossypimine
Safranine O,certified
Safranin
EINECS 207-518-8
Hidaco safranine
MFCD00011759
Safranine O
Safranin O
Tolusafranine
Safranine O,high purity biological stain
Cotton Red
Safranin Y or A
Safranine-O chloride
Safranine T
Basic Red 2,Cotton Red,Gossypimine
Description Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration[1].
Related Catalog
References

[1]. Wan H, et al. Structure characterization and optical properties investigation of the four main components of the classical phenazinium dye Safranin O. Analyst. 2019;144(24):7149-7156.

Density 1.00 g/mL at 20 °C
Molecular Formula C20H19ClN4
Molecular Weight 350.845
Flash Point 46 °C
Exact Mass 350.129822
PSA 68.81000
LogP 1.61230
Index of Refraction n20/D 1.339
Stability Stable.

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SG1623000
CHEMICAL NAME :
Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride
CAS REGISTRY NUMBER :
477-73-6
LAST UPDATED :
199701
DATA ITEMS CITED :
11
MOLECULAR FORMULA :
C20-H19-N4.Cl
MOLECULAR WEIGHT :
350.88

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
28740 ug/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
1600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
24020 ug/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
26940 ug/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes

MUTATION DATA

TYPE OF TEST :
DNA repair
TEST SYSTEM :
Bacteria - Bacillus subtilis
DOSE/DURATION :
2 mg/disc
REFERENCE :
TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1- 1949/50- Volume(issue)/page/year: 27,153,1976 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1441 No. of Facilities: 1027 (estimated) No. of Industries: 9 No. of Occupations: 18 No. of Employees: 4314 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1441 No. of Facilities: 1253 (estimated) No. of Industries: 6 No. of Occupations: 10 No. of Employees: 22263 (estimated) No. of Female Employees: 14330 (estimated)
Symbol GHS05
GHS05
Signal Word Danger
Hazard Statements H318
Precautionary Statements P280-P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi:Irritant;
Risk Phrases R36/38
Safety Phrases 26-39-24/25-36-16
RIDADR UN 1170 3/PG 3
WGK Germany 3
RTECS SG1623000
HS Code 32041300
95-53-4 structure

95-53-4

~%

477-73-6 structure

477-73-6
Literature: Chemische Berichte, , vol. 28, p. 273 Chemische Berichte, , vol. 16, p. 466 Kuenstliche organische Farbstoffe , S.323. Grundlegende Operationen der Farbenchemie, 3.Aufl. , S.192 DE61692 ; Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 3, p. 794 DE108497 ; Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 5, p. 540 Chemische Berichte, , vol. 13, p. 207 Chemische Berichte, , vol. 16, p. 864 Chemische Berichte, , vol. 5, p. 527 Chemische Berichte, , vol. 12, p. 937,939 Chemische Berichte, , vol. 10, p. 874
95-70-5 structure

95-70-5

~%

477-73-6 structure

477-73-6
Literature: Zhurnal Russkago Fiziko-Khimicheskago Obshchestva, , vol. 42, p. 945 Chem. Zentralbl., , vol. 81, # II p. 1761
3029-60-5 structure

3029-60-5
6406-71-9 structure

6406-71-9

~%

477-73-6 structure

477-73-6
Literature: Zhurnal Russkago Fiziko-Khimicheskago Obshchestva, , vol. 42, p. 945 Chem. Zentralbl., , vol. 81, # II p. 1761
HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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title: CAS No. 477-73-6 | Chemsrc address: https://www.chemsrc.com/en/baike/244097.html

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