Myosin light chain kinase (MLCK) is a ubiquitous Ca2+/calmodulin (CaM)-activated kinase found in smooth, cardiac, and skeletal muscle as well as in mammalian nonmuscle cells. Myosin light chain kinase (MLCK) is a regulatory protein forsmooth muscle contraction, which acts by phosphorylating 20-kDamyosin light chain (MLC20) to activate the myosin ATPase activity.Myosin light chain kinase (MLCK) of smooth muscle hasbeen puried as an enzyme that phosphorylates 20-kDa lightchain of smoothmuscle myosin (MLC20). Analysis of the cross talk between Ras-ERK and PI3K-AKT signaling pathways reveals integrin β1, myosin light chain kinase (MLCK) and myosin IIA are required for the activation of PI3K-AKT following inhibition of the Ras-ERK pathway. Integrin β1, MLCK, and myosin IIA are factors in the development of resistance to MEK inhibitors. Myosin light chain kinase (MLCK) phosphorylates the regulatory light chain (RLC) of myosin producing increases in force development during skeletal muscle contraction.


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ML-7 hydrochloride

ML-7 hydrochloride is a naphthalene sulphonamide derivative, potently inhibits MLCK (IC50=300 nM) and TRPC6 channel (IC50>10 μM).

  • CAS Number: 110448-33-4
  • MF: C15H18ClIN2O2S
  • MW: 452.738
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: 542.7ºC at 760 mmHg
  • Melting Point: 246-249ºC dec.
  • Flash Point: 282ºC

(-)-Blebbistatin

(-)-Blebbistatin is an S enantiomer of blebbistatin. Blebbistatin is a potent and selective myosin II inhibitor with IC50s ranging from 0.5 to 5 μM.

  • CAS Number: 856925-71-8
  • MF: C18H16N2O2
  • MW: 292.33
  • Catalog: Myosin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 486.7±55.0 °C at 760 mmHg
  • Melting Point: 210-212ºC
  • Flash Point: 248.1±31.5 °C

ML-9 Free Base

ML-9 (Free Base) is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity[3]. ML-9 (Free Base) inhibits inhibits MLCK, PKA and PKC activity with Ki values of 4, 32 and 54 μM, respectively[1]. ML-9 (Free Base) induces autophagy by stimulating autophagosome formation and inhibiting their degradation[3].

  • CAS Number: 110448-31-2
  • MF: C15H17ClN2O2S
  • MW: 324.82600
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Mavacamten

Mavacamten is a modulator of cardiac myosin, with IC50s of 490, 711 nM for bovine cardiac and human cardiac, respectively.

  • CAS Number: 1642288-47-8
  • MF: C15H19N3O2
  • MW: 273.330
  • Catalog: Myosin
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ATM-3507

ATM-3507 is a potent tropomyosin inhibitor with IC50s from 3.83-6.84 μM in human melanoma cell lines.

  • CAS Number: 1861449-70-8
  • MF: C37H46FN5O2
  • MW: 611.79
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Mavacamten-d1

Mavacamten-d1 (MYK461-d1; SAR439152-d1) is deuterium labeled Mavacamten (HY-109037). Mavacamten (MYK461) is an orally active modulator of cardiac myosin, with IC50s of 490, 711 nM for bovine cardiac and human cardiac, respectively.

  • CAS Number: 2453251-02-8
  • MF: C15H18DN3O2
  • MW: 274.34
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Danicamtiv

Danicamtiv is a positive inotropic agent.

  • CAS Number: 1970972-74-7
  • MF: C16H20F3N5O4S
  • MW: 435.422
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Mavacamten-d5

Mavacamten-d5 (MYK461-d5; SAR439152-d5) is deuterium labeled Mavacamten (HY-109037). Mavacamten (MYK461) is an orally active modulator of cardiac myosin, with IC50s of 490, 711 nM for bovine cardiac and human cardiac, respectively.

  • CAS Number: 2453251-00-6
  • MF: C15H14D5N3O2
  • MW: 278.36
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Myosin Light Chain Kinase Substrate (smooth muscle)

Myosin Light Chain Kinase Substrate (smooth muscle) is a smooth muscle myosin light chain kinase (MLCK) synthetic peptide substrate[1].

  • CAS Number: 119386-41-3
  • MF: C61H107N23O16
  • MW: 1418.65
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MPH-220

MPH-220 is a selective and orally active inhibitor of skeletal muscle myosin-2. MPH-220 enables muscle relaxation. MPH-220 is anti-spastic agent that can be used in the research of spasticity and muscle stiffness[1].

  • CAS Number: 2649776-79-2
  • MF: C20H21N3O3S
  • MW: 383.46
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pentachloropseudilin

Pentachloropseudilin (Antibiotic A 15104 Y; PClP) is a reversible and allosteric potent inhibitor of Myo1s (class 1 myosins) with IC50s range from 1 to 5 μM for mammalian class-1 myosins and greater than 90 μM for class-2 and class-5 myosins. Pentachloropseudilin is a potent inhibitor of transforming growth factor-β (TGF-β)-stimulated signaling, with an IC50 of 0.1 to 0.2 μM for TGF-β[1][2].

  • CAS Number: 69640-38-6
  • MF: C10H4Cl5NO
  • MW: 331.41
  • Catalog: Myosin
  • Density: 1.733g/cm3
  • Boiling Point: 445.9ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 223.5ºC

Myosin V-IN-1

Myosin V-IN-1 (compound 8) is a potent and selective Myosin V inhibitor, with a Ki of 6 μM. Myosin V-IN-1 shows acute inhibition of myosin V. Myosin V-IN-1 slows the actin-activated myosin V ATPase by specifically inhibiting ADP release from the actomyosin complex[1][2].

  • CAS Number: 1259177-59-7
  • MF: C29H26N6O3S
  • MW: 538.62
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JB002

JB002 is a myosin II inhibitor, with an IC50 of ≤10 μM. JB002 can be used for the research of muscle spasticity, chronic musculoskeletal pain, and hypertrophic cardiomyopathy[1].

  • CAS Number: 30408-07-2
  • MF: C18H15NO3
  • MW: 293.32
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

W-7 hydrochloride

W-7 hydrochloride is a selective calmodulin antagonist. W-7 hydrochloride inhibits the Ca2+-calmodulin-dependent phosphodiesterase and myosin light chain kinase with IC50 values of 28 μM and 51 µM, respectively[1][2]. W-7 hydrochloride induces apoptosis and has antitumor activity[3].

  • CAS Number: 61714-27-0
  • MF: C16H22Cl2N2O2S
  • MW: 377.329
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: 518.8ºC at 760 mmHg
  • Melting Point: 220-222 °C
  • Flash Point: 267.6ºC

Sevasemten

Sevasemten is an allosteric inhibitor of skeletal muscle myosin. Sevasemten exhibits selectively myosin inhibition with IC50s of ≤10 μM (skeletal), >100 μM (cardiac), respectively[1][2].

  • CAS Number: 2417395-15-2
  • MF: C16H11F4N5O2
  • MW: 381.28
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BTS

BTS is a potent inhibitor of Ca2+-stimulated myosin S1 ATPase (IC50 ~ 5 μM) and reversibly blocks the gliding motility. IC50 value: 5 uMTarget: Ca2+-stimulated myosin S1 ATPaseBTS also weakens myosin’s interaction with F-actin. BTS is much less effective in suppressing contraction in rat myocardial or rabbit slow twitch muscle and has no effect on platelet myosin II.

  • CAS Number: 1576-37-0
  • MF: C14H15NO2S
  • MW: 261.33900
  • Catalog: Myosin
  • Density: 1.204 g/cm3
  • Boiling Point: 419.1ºC at 760 mmHg
  • Melting Point: 115 °C
  • Flash Point: 207.3ºC

Mavacamten-d6

Mavacamten-d6 (MYK461-d6; SAR439152-d6) is deuterium labeled Mavacamten (HY-109037). Mavacamten (MYK461) is an orally active modulator of cardiac myosin, with IC50s of 490, 711 nM for bovine cardiac and human cardiac, respectively.

  • CAS Number: 2453251-18-6
  • MF: C15H13D6N3O2
  • MW: 279.37
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MLCK inhibitor peptide 18

MLCK inhibitor peptide 18 is a myosin light chain kinase (MLCK) inhibitor with an IC50 of 50 nM, and inhibits CaM kinase II only at 4000-fold higher concentrations.

  • CAS Number: 224579-74-2
  • MF: C60H105N23O11
  • MW: 1324.62000
  • Catalog: Peptides
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

THYMOSIN BETA4 (16-38)

16-38-Thymosin β4 (cattle) is a Ca2+-independent MLCK activator with high affinity.

  • CAS Number: 113318-05-1
  • MF: C118H204N32O41
  • MW: 2727.072
  • Catalog: Myosin
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 2127.1±75.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 1240.2±37.1 °C

para-Nitroblebbistatin

para-Nitroblebbistatin is a non-cytotoxic, photostable, fluorescent and specific Myosin II inhibitor, usd in the study of the specific role of myosin II in physiological, developmental, and cell biological studies[1].

  • CAS Number: 1621326-32-6
  • MF: C18H15N3O4
  • MW: 337.33
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

(S)-(-)-Blebbistatin

Blebbistatin is a selective non-muscle myosin II (NMII) inhibitor, promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function[1].

  • CAS Number: 674289-55-5
  • MF: C18H16N2O2
  • MW: 292.332
  • Catalog: Myosin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 507.3±60.0 °C at 760 mmHg
  • Melting Point: 210-212ºC
  • Flash Point: 260.6±32.9 °C

Omecamtiv mecarbil (CK-1827452)

Omecamtiv mecarbil is a cardiac myosin activator.

  • CAS Number: 873697-71-3
  • MF: C20H24FN5O3
  • MW: 401.435
  • Catalog: Myosin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 456.8±45.0 °C at 760 mmHg
  • Melting Point: 180℃
  • Flash Point: 230.1±28.7 °C

HA-100 hydrochloride

HA-100 hydrochloride is a potent protein kinase inhibitor, with IC50s of 4 μM, 8 μM, 12 μM and 240 μM for cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and MLC-kinase, respectively. HA-100 hydrochloride also used as a ROCK inhibitor[1][2].

  • CAS Number: 141543-63-7
  • MF: C13H16ClN3O2S
  • MW: 313.80300
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: 497.4ºC at 760 mmHg
  • Melting Point: 252-254ºC
  • Flash Point: 254.6ºC

HA-100 hydrochloride

HA-100 dihydrochloride is a potent protein kinase inhibitor, with IC50s of 4 μM, 8 μM, 12 μM and 240 μM for cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and MLC-kinase, respectively. HA-100 dihydrochloride also used as a ROCK inhibitor[1][2].

  • CAS Number: 210297-47-5
  • MF: C13H17Cl2N3O2S
  • MW: 350.26
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JB062

JB062 is a nonmuscle myosin inhibitor with IC50 values of 1.6, 5.4, and >100 μM for Skeletal muscle myosin, Cardiac muscle myosin, and Smooth muscle myosin II, respectively. JB062 has cytotoxic to human cancer cells but not normal cells. JB062 can be used in research of muscle spasticity, chronic musculoskeletal pain, and hypertrophic cardiomyopathy[1].

  • CAS Number: 2417988-00-0
  • MF: C19H17NO4
  • MW: 323.34
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MS-444

MS-444 inhibits the activity of purified smooth muscle myosin light chain kinase (MLCK) with an IC50 value of 10 μM.

  • CAS Number: 150045-18-4
  • MF: C13H10O4
  • MW: 230.21600
  • Catalog: Myosin
  • Density: 1.452g/cm3
  • Boiling Point: 448.7ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 225.2ºC

(+)-Blebbistatin

(+)-Blebbistatin is the inactive enantiomer of (–)-Blebbistatin. (–)-Blebbistatin is a selective inhibitor of myosin II ATPase[1].

  • CAS Number: 1177356-70-5
  • MF: C18H16N2O2
  • MW: 292.332
  • Catalog: Myosin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 507.3±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 260.6±32.9 °C

HA-100

HA-100 is an inhibitor of cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), Protein kinase C (PKC) and MLC-kinase with IC50s of 4, 8, 12 and 240 μM, respectively.

  • CAS Number: 84468-24-6
  • MF: C13H15N3O2S
  • MW: 277.34200
  • Catalog: Myosin
  • Density: 1.332g/cm3
  • Boiling Point: 497.4ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 254.6ºC

Aficamten

Aficamten (CK-274) is a novel cardiac myosin inhibitor with an IC50 of 1.4 μM. Aficamten can be used for the research of hypertrophic cardiomyopathy (HCM)[1].

  • CAS Number: 2364554-48-1
  • MF: C18H19N5O2
  • MW: 337.38
  • Catalog: Myosin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

para-amino-Blebbistatin

Para-aminoblebbistatin is a highly water soluble, non-fluorescent and photostable C15 amino-substituted derivative of blebbistatin; inhibits various (myosin II) isoforms both in vitro and in vivo.

  • CAS Number: 2097734-03-5
  • MF: C18H17N3O2
  • MW: 307.346
  • Catalog: Myosin
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 571.3±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 299.3±32.9 °C