Apigenin

Apigenin Structure
Apigenin
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Common Name Apigenin
CAS Number 520-36-5 Molecular Weight 270.237
Density 1.5±0.1 g/cm3 Boiling Point 555.5±50.0 °C at 760 mmHg
Molecular Formula C15H10O5 Melting Point >300 °C(lit.)
MSDS Chinese USA Flash Point 217.1±23.6 °C
Symbol GHS07
GHS07
Signal Word Warning

 Use of Apigenin


Apigenin is a competitive CYP2C9 inhibitor with a Ki of 2 μM.

 Names

Name apigenin
Synonym More Synonyms

 Apigenin Biological Activity

Description Apigenin is a competitive CYP2C9 inhibitor with a Ki of 2 μM.
Related Catalog
Target

Ki: 2 μM (CYP2C9)[1]

In Vitro Apigenin inhibits cytochrome P450 2C9 (CYP2C9) with a Ki of 2 μM in the CYP2C9 RECO system (a purified, reconstituted enzyme system containing recombinant human CYP2C9, P450 reductase, cytochrome b5, and liposomes)[1]. Apigenin inhibits the cell proliferation. The growth inhibition rate (IR) of 20, 40, and 80 μM of Apigenin is 38%, 71%, and 99% respectively on the 7thd. after exposure to Apigenin for 24 or 48 h, the clone formation of SGC-7901 cells is suppressed in a dose- and time-dependent manner. The cloning efficiency in 80 μM is 9.8% and 5% after treatment with Apigenin for 24 and 48 h, while in the control group it is 40.4% and 43.4%[2].
In Vivo Apigenin (API), a natural flavonoid, possesses a broad spectrum of biological properties, including antioxidative, anti-inflammatory, anticancer, and neuroprotective effects. Apigenin (125 mg/kg and 250  mg/kg) alleviates Adriamycin (ADR) (24 mg/kg)-induced myocardial injury. Apigenin inhibits serum aspartate amino transferase (AST) release. Apigenin reduces serum lactate dehydrogenase (LDH) release. Apigenin reduces serum creatine kinase (CK) contents[3].
Solvent
In Vitro:

DMSO : ≥ 30 mg/mL (111.01 mM)

* "≥" means soluble, but saturation unknown.

Solubility
1 mM 3.7004 mL 18.5021 mL 37.0041 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL
10 mM 0.3700 mL 1.8502 mL 3.7004 mL
Cell Assay The effect of Apigenin on the viability of cells is determined by MTT assay. Near-confluent stock cultures of human gastric cancer SGC-7901 cells are harvested with 0.2% EDTA and plated at a density of 2.5×103/well in 96-well microtiter plates. After an overnight incubation to allow cell attachment, the medium is replaced by fresh medium containing different concentrations (0, 20, 40, and 80 μM) of Apigenin. Control wells receive DMSO (0.2%). Each concentration of Apigenin is repeated in four wells. After incubation for 24 h, one plate is assayed with a microplate reader at the wavelength of 570 nm. Before the assay, MTT (5 mg/mL in PBS) is added to each well and incubated for 4 h, then MTT solution is removed from the wells by aspiration. After careful removal of the medium, 0.1 mL of DMSO is added to each well, and the plate is shaken for 15 min. The data of 7 d are fed into the computer and the growth curve is drawn. The growth inhibition rate (IR) is calculated[2].
Animal Admin Mice[3] Sixty healthy Kunming mice (26±2 g) are randomly assigned into two groups: a control group (n=15) and an ADR group (n=45). The ADR group is divided into three subgroups: ADR only without Apigenin (ADR, n=15), low-dose Apigenin (125 mg/kg/day, n=15), and high-dose Apigenin (250 mg/kg/day, n=15). All Apigenin-treated groups are treated daily via gastric gavage for seventeen days with a 125 or 250 mg/kg/day dose. ADR (3 mg/kg/day) is injected intraperitoneally into animals at an interval of 48 h (in total, eight times at a cumulative dose of 24 mg/kg). The mice in the control group receive injections of 0.9% sterile saline. On the 17th day after the first treatment, the mice are sacrificed, and blood samples are collected. A number of hearts are fixed with 2.5% glutaraldehyde fixative for electron microscopy analysis, and the others are stored at -80°C for western blot analysis.
Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping Room temperature in continental US; may vary elsewhere
SMILES OC1=C2C(OC(C3=CC=C(O)C=C3)=CC2=O)=CC(O)=C1
References

[1]. Si D, et al. Mechanism of CYP2C9 inhibition by flavones and flavonols. Drug Metab Dispos. 2009 Mar;37(3):629-34.

[2]. Wu K, et al. Inhibitory effects of apigenin on the growth of gastric carcinoma SGC-7901 cells. World J Gastroenterol. 2005 Aug 7;11(29):4461-4.

[3]. Yu W, et al. Apigenin Attenuates Adriamycin-Induced Cardiomyocyte Apoptosis via the PI3K/AKT/mTOR Pathway. Evid Based Complement Alternat Med. 2017;2017:2590676.

Related Molecules Talarozole | Cobicistat | Ginsenoside C-K | Gemfibrozil | Isavuconazole | Naringin | Orteronel | Proadifen hydrochloride | Galangin | Galeterone | Tetrahydrocurcumin | 1-Aminobenzotriazole | furafylline | Ginsenoside F1 | Schisandrin A
Related Doc

SDS |COA |HNMR

*The above documents are provided by Medchemexpress and are for scientific research only.

 Chemical & Physical Properties

Density 1.5±0.1 g/cm3
Boiling Point 555.5±50.0 °C at 760 mmHg
Melting Point >300 °C(lit.)
Molecular Formula C15H10O5
Molecular Weight 270.237
Flash Point 217.1±23.6 °C
Exact Mass 270.052826
PSA 90.90000
LogP 2.10
Vapour Pressure 0.0±1.6 mmHg at 25°C
Index of Refraction 1.732
Water Solubility DMSO: 27 mg/mL

 Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
LK9276000
CHEMICAL NAME :
Flavone, 4',5,7-trihydroxy-
CAS REGISTRY NUMBER :
520-36-5
BEILSTEIN REFERENCE NO. :
0262620
LAST UPDATED :
199707
DATA ITEMS CITED :
3
MOLECULAR FORMULA :
C15-H10-O5
MOLECULAR WEIGHT :
270.25
WISWESSER LINE NOTATION :
T66 BO EVJ CR DQ& GQ IQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :
Sister chromatid exchange
TEST SYSTEM :
Human Lymphocyte
DOSE/DURATION :
10 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 246,205,1991

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319-H335
Precautionary Statements P261-P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi:Irritant
Risk Phrases R36/37/38
Safety Phrases S26-S36
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS LK9276000
HS Code 2942000000

 Customs

HS Code 2914501900
Summary 2914501900 other ketone-phenols。Supervision conditions:None。VAT:17.0%。Tax rebate rate:9.0%。MFN tariff:5.5%。General tariff:30.0%

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