OSMI-2
Modify Date: 2025-08-25 11:57:46
OSMI-2 structure
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Common Name | OSMI-2 | ||
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| CAS Number | 2260542-60-5 | Molecular Weight | 555.62 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C26H25N3O7S2 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of OSMI-2OSMI-2 (Compound 1b) is a cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-2 increases detained intron splicing in cells[1]. |
| Name | OSMI-2 |
|---|
| Description | OSMI-2 (Compound 1b) is a cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-2 increases detained intron splicing in cells[1]. |
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| Related Catalog | |
| Target |
O-linked N-acetylglucosamine transferase (OGT)[1] |
| In Vitro | OSMI-2 (Compound 1b; 20-50 μM; 4-24 hours; HCT116 cells) treatment reduces O-GlcNAc levels at short treatment times (<8h). However, O-GlcNAc levels begins to recover at longer treatment times with OSMI-2[1].A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 in cells treated with OSMI-2 (Compound 1b) is observed. Because OGT knockdown is known to decrease cell proliferation, the effects of OSMI-2 on cell growth in culture over 96h is also monitored. Although there is no evidence of apoptosis, the reduced growth of cells over time is observed, consistent with the knockdown results[1]. A time course performed with 20 μM OSMI-2 (Compound 1b) shows reciprocal changes in the abundance of OGT and OGA, with a particularly large increase in OGT; this may explain the recovery in O-GlcNAc levels with OSMI-2. At 24h where O-GlcNAc levels fully recovered[1]. Western Blot Analysis[1] Cell Line: HCT116 cells Concentration: 20 μM, 40 μM, 50 μM Incubation Time: 4 hours, 24 hours Result: Reduced O-GlcNAc levels. |
| References |
| Molecular Formula | C26H25N3O7S2 |
|---|---|
| Molecular Weight | 555.62 |