| In Vivo |
Epoetin beta (rhEPO) (1000 IU/kg; s.c.; three times per week over 14 days) prevents anaemia and enhances the radiosensitivity of solid growing DS-sarcomas[2]. Animal Model: Male Sprague-Dawley rats, body weight 140-170 g[2] Dosage: 1000 IU/kg Administration: Subcutaneous injection, three times per week over 14 days starting 9 days before tumour implantation. Solid DS-sarcomas were induced by injecting DS-sarcoma cells (0.4 mL, approximately 104 cells/uL) subcutaneously into the hind food dorsum. A prolonged anaemia was induced in all animals by a single i.v. dose of carboplatin (45 mg/kg dissolved in isotonic saline at a concentration of 20 mg/mL) into the tail vein 3 days before tumour implantation. Result: Prevented anaemia and significantly increased the radiosensitivity of solid growing DS-sarcomas, tumours showing pronounced hypoxia even under non-anaemic control conditions.
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