In Vitro |
Zoledronic Acid disodium tetrahydrate (0.1-1 µM; 48 hours) increases receptor activator of nuclear factor kB ligand (RANKL) and sclerostin mRNA expressions in osteocyte-like MLO-Y4 cells[2]. Zoledronic Acid disodium tetrahydrate increases the expression of osteoclastogenesis supporting factor from MLO-Y4 cells[2]. Zoledronic Acid disodium tetrahydrate enhances the RANKL expression via IL-6/ JAK2/STAT3 pathway in MLO-Y4 cells[2]. Zoledronic acid disodium tetrahydrate inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways[3]. Zoledronic Acid disodium tetrahydrate (10-100 µM; 1-7 days) markedly reduces the viability of MC3T3-E1 cells and induces apoptosis in MC3T3-E1 cells[4]. Zoledronic Acid disodium tetrahydrate (10-100 µM; 4 days) inhibits cell viability due to the induction of apoptosis[4]. Zoledronic Acid disodium tetrahydrate exerts inhibitory effects on the differentiation and maturation of MC3T3-E1 cells at concentrations <1 µM[4]. Cell Viability Assay[4] Cell Line: MC3T3-E1 cells Concentration: 0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM Incubation Time: 1 day, 3 days, 5 days, 7 days Result: Reduced cells viability at 10 µM and 100 µM. Apoptosis Analysis[4] Cell Line: MC3T3-E1 cells Concentration: 0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM Incubation Time: 1 days, 4 days, 7 days Result: Increased the number of early apoptotic cells and late apoptotic or necrotic cells at dose-dependent and time-dependent (high concentrations). Western Blot Analysis[4] Cell Line: MC3T3-E1 cells Concentration: 0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM Incubation Time: 4 days Result: Down-regulated the protein level of inactive caspase-3 and up-regulated the protein level of active caspase-3 at the concentrations of 10 and 100 µM.
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