136171-87-4
136171-87-4 structure
- Name: Lobetyol
- Chemical Name: tetradeca-4,12-dien-8,10-diyne-1,6,7-triol
- CAS Number: 136171-87-4
- Molecular Formula: C14H18O3
- Molecular Weight: 234.29100
- Catalog: Signaling Pathways Apoptosis Apoptosis
- Create Date: 2016-07-03 06:26:20
- Modify Date: 2025-08-25 19:27:51
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Lobetyol is a natural compound that can be isolated from Lobelia chinensis. Lobetyol induces apoptosis and cell cycle arrest in MKN45 cells. Lobetyol shows anti-virus, anti-inflammation and anti-tumor activity[1][2].
| Name | tetradeca-4,12-dien-8,10-diyne-1,6,7-triol |
|---|---|
| Synonyms |
4,12-Tetradecadiene-8,10-diyne-1,6,7-triol
Lobetyol |
| Description | Lobetyol is a natural compound that can be isolated from Lobelia chinensis. Lobetyol induces apoptosis and cell cycle arrest in MKN45 cells. Lobetyol shows anti-virus, anti-inflammation and anti-tumor activity[1][2]. |
|---|---|
| Related Catalog | |
| Target |
Bax Bcl-2 |
| In Vitro | Lobetyol (0-100 μg/mL, 0-48 h) 在 MKN45 细胞中导致剂量和时间依赖性增殖抑制[1]。 Lobetyol (0-100 μg/mL, 48 h) 以时间和剂量依赖的方式诱导 MKN45 细胞细胞凋亡和细胞周期停滞[1]。 Lobetyol (0-100 μg/mL, 48 h) 增加 Bax、P53 的表达水平，降低 Bcl-2 的表达水平[1]。 Cell Proliferation Assay[1] Cell Line: MKN45 cells Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time: 12 h, 24 h, 48 h Result: Showed a dose-and time-dependent proliferation inhibition in MKN45 cells, with an IC50 of 71.47 ± 4.29 μg/mL at 48 h. Exhibited more safety on L02 and HEK293 human normal cell lines. Apoptosis Analysis[1] Cell Line: MKN45 cells Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time: 48 h Result: Increased the apoptotic population in MKN45 cells in a dose-dependent manner. Flow cytometic assay of Annexin-V/PI revealed apoptotic populations under increasing dose of lobetyol ranging from 0 μg/mL, 50 μg/mL, 75 μg/mL, to 100 μg/mL are 5.5%, 13.74%, 27.32% to 31.57%, respectively. Western Blot Analysis[1] Cell Line: MKN45 cells Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time: 12 h, 24 h, 48 h Result: Increased the expression level of Bax, P53, and decreased the expression level of Bcl-2. The release of cytochrome c was detected, followed by caspase-9 and -3 activations. |
| In Vivo | Lobetyol (0-10 mg/kg，每3天一次)抑制 MKN45 裸鼠模型中的肿瘤生长[1]。 Animal Model: BALB/C nude mice (6 weeks-old, injected intraperitoneally with MKN45 cells)[1] Dosage: 0, 2, 5, 10 mg/kg Administration: Once per 3 days Result: Suppressed tumor growth in MKN45 nude models. The protein levels of Ki67 in MKN45 cells were significantly decreased. Meanwhile, cleaved-caspase-3 positive MKN45 cells were found increased with the tendency of lobetyol concentrations in the study in vivo. |
| References |
| Density | 1.133±0.06 g/cm3(Predicted) |
|---|---|
| Boiling Point | 468.3±45.0 °C(Predicted) |
| Molecular Formula | C14H18O3 |
| Molecular Weight | 234.29100 |
| Exact Mass | 234.12600 |
| PSA | 60.69000 |
| LogP | 0.61980 |