| In Vitro |
Pomstafib-2 (compound 8) (0-10 µM; 4 h) decreases the expression of pSTAT5b in a dose-dependent manner in STAT5b-GFP-transfected K562 cells[1]. Pomstafib-2 (0, 10, 20, 40, 60, 80 µM; 48 h) induces apoptosis in a dose-dependent manner in STAT5-dependent K562 cells[1]. Western Blot Analysis[1] Cell Line: K562 leukaemia cells Concentration: 0, 0.3, 1, 3, 10 µM Incubation Time: 4 h Result: Decreased the expression of pSTAT5b in a dose-dependent manner. Apoptosis Analysis[1] Cell Line: K562, MDA-MB-231 cells Concentration: 0, 10, 20, 40, 60, 80 µM Incubation Time: 48 h Result: Induced apoptosis in a dose-dependent manner in K562 cells, does not increased the apoptotic rate of STAT5-independent MDA-MB-231 cells.
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