914389-14-3
914389-14-3 structure
- Name: SMP-028
- Chemical Name: SMP-028
- CAS Number: 914389-14-3
- Molecular Formula: C23H26FN5O2S
- Molecular Weight: 455.55
- Catalog: Research Areas Metabolic Disease
- Create Date: 2019-03-03 15:24:03
- Modify Date: 2024-01-09 19:39:37
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SMP-028 is an inhibitor of neutral cholesterol esterase (CEase), with an IC50 of 1.01 μM.
| Name | SMP-028 |
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| Description | SMP-028 is an inhibitor of neutral cholesterol esterase (CEase), with an IC50 of 1.01 μM. |
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| Related Catalog | |
| Target |
IC50: 1.01 μM (CEase)[1]. |
| In Vitro | SMP-028 potently and concentration-dependently inhibits neutral cholesterol esterase (CEase) with an IC50 value of 1.01 μM. On the other hand, inhibition of other steroidogenic enzymes by SMP-028 is weak with IC50 values>10 μM. In particular, SMP-028 does not inhibit acid CEase and only weakly reduces the activity of CYP11A1 (IC50 values>100 μM and 49.8 μM respectively) [1]. SMP-028 at 10 μM or less does not affect the viability of male adrenal cells, female adrenal cells, testicular cells, and ovarian cells. On the other hand, SMP-028 at concentrations higher than 30 μM significantly reduce the viability of male adrenal cells, female adrenal cells [2]. |
| Cell Assay | Testicular and ovarian cells from Sprague–Dawley rats are cultured in medium containing SMP-028 dissolved in dimethyl sulfoxide (DMSO) is added to each well of the 96-well cell culture plates. The final concentration of DMSO is 0.1% (v/v) and that of SMP-028 is set between 0.1 μM to 50 μM. The cells with culture medium containing DMSO only (not containing SMP-028) are as a control. The plates are next incubated at 37°C, 5% CO2 in air atmosphere for 24 hours. SMP-028 cytotoxicity is estimated. The luminescence of each plate is measured[2]. |
| References |
| Molecular Formula | C23H26FN5O2S |
|---|---|
| Molecular Weight | 455.55 |