DC Chemicals Limited
China
Product Name: DO-34
Price: $Inquiry/100mg $Inquiry/250mg $Inquiry/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

1848233-58-8

1848233-58-8 structure

1848233-58-8 structure

Name: DO34
Chemical Name: DO34
CAS Number: 1848233-58-8
Molecular Formula: C₂₆H₂₈F₃N₅O₄
Molecular Weight: 531.53
Catalog: Research Areas Metabolic Disease
Create Date: 2019-02-08 12:55:22
Modify Date: 2024-01-11 04:50:37
DO34 is a highly potent, selective and centrally active diacylglycerol lipase (DAGL) inhibitor, with an IC50 of 6 nM for DAGLα conversion of SAG to 2-AG, and an IC50 for DAGLβ.

Name	DO34

Description	DO34 is a highly potent, selective and centrally active diacylglycerol lipase (DAGL) inhibitor, with an IC50 of 6 nM for DAGLα conversion of SAG to 2-AG, and an IC50 for DAGLβ.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Metabolic Disease
Target	IC50: 6 nM (DAGLα), 3-8 nM (DAGLβ)[1].
In Vitro	DO34 is a highly potent, selective and centrally active DAGL inhibitor, with an IC50 of 6 nM for DAGLα conversion of SAG to 2-AG, as determined using a real-time, fluorescence-based natural substrate assay with membrane lysates from HEK293T cells expressing recombinant human DAGLα. It is also confirmed that and DO34 is a potent inhibitor of DAGLβ with IC50 of 3-8 nM[1].
In Vivo	DO34 (compound 39) prevents fasting-induced refeeding of mice, which is typical cannabinoid CB1-receptor mediated behavior. DO34 (comound 39) reduces brain 2-AG levels in dose- and time dependent manner[2]. DO34 could block the tonic CB1 activation. AM251 significantly increases basal PF-EPSCs in MAGL-TKO mice, and the effect of AM251 is blocked by the DAGL inhibitor DO34[3].
Animal Admin	Mice[2] 12-week-old male C57Bl/6J mice are single housed in metabolic cages with a regular 12:12h light/dark cycle (6 a.m.-6 p.m.) and free access to food and water unless noted otherwise. After 3 days of acclimatization, mice are fasted for 18h starting at midnight followed by an intraperitoneal injection with 38 (50 mg/kg), DO34 (compound 39: 50 mg/kg), 40 (50 mg/kg) or vehicle 30 min prior to refeeding[2].
References	[1]. Ogasawara D, et al. Rapid and profound rewiring of brain lipid signaling networks by acute diacylglycerol lipase inhibition. Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):26-33. [2]. Deng H, et al. Triazole Ureas Act as Diacylglycerol Lipase Inhibitors and Prevent Fasting-Induced Refeeding. J Med Chem. 2017 Jan 12;60(1):428-440. [3]. Liu X, et al. Coordinated regulation of endocannabinoid-mediated retrograde synaptic suppression in the cerebellum by neuronal and astrocytic monoacylglycerol lipase. Sci Rep. 2016 Oct 24;6:35829.

Molecular Formula	C₂₆H₂₈F₃N₅O₄
Molecular Weight	531.53
Storage condition	2-8℃