1445846-30-9
1445846-30-9 structure
- Name: GPR84 antagonist 8
- Chemical Name: GPR84 antagonist 8
- CAS Number: 1445846-30-9
- Molecular Formula: C23H23N3O5
- Molecular Weight: 421.45
- Catalog: Signaling Pathways GPCR/G Protein GPR84
- Create Date: 2019-01-20 12:20:21
- Modify Date: 2025-08-27 11:43:23
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GPR84 antagonist 8 is a selective GPR84 antagonist.
| Name | GPR84 antagonist 8 |
|---|
| Description | GPR84 antagonist 8 is a selective GPR84 antagonist. |
|---|---|
| Related Catalog | |
| Target |
GPR84[1] |
| In Vitro | GPR84 is a member of the metabolic G protein-coupled receptor family, and its expression has been described predominantly in immune cells. To test the hypothesis that blocking the activation of GPR84 can be a potential anti-inflammatory strategy in different inflammatory diseases, GPR84 antagonist 8 is used. The potency and specificity of GPR84 antagonist 8 is assessed tusing GPR84-CHO cells in the cAMP assay. GPR84 antagonist 8 effectively inhibits the action of 6-OAU in decreasing cAMP production in GPR84-CHO cells. To test GPR84 antagonist 8’s inhibition of the pro-inflammatory effects of GPR84 activation in macrophages, LPS pre-treated BMDMs are incubated with 10 µM GPR84 antagonist 8 for 30 min before adding 1 µM 6-OAU. Protein analysis by Western Blot shows that the GPR84 antagonist 8 partially blocks the phosphorylation of AKT and ERK induced by 6-OAU[1]. |
| Cell Assay | Bone marrow-derived macrophages treated with either vehicle (0.3% DMSO) or 1 µM 6-OAU for 1 h are incubated with unopsonised pHrodo E. coli bioparticles at 0.1 mg/mL in a 96-well flat clear bottom plate. For the inhibition studies with GPR84 antagonist 8, cells are pretreated with 10 µM GPR84 antagonist 8 for 30 min before addition of either vehicle or 6-OAU. The plate is then placed into the IncuCyte Zoom platform which is housed inside a humidified incubator at 37°C, 5% CO2. Two to four images per well from three technical replicates are taken every 15 min for 4 h[1]. |
| References |
| Molecular Formula | C23H23N3O5 |
|---|---|
| Molecular Weight | 421.45 |
| Storage condition | 2-8℃ |