878451-87-7
878451-87-7 structure
- Name: RS-246204
- Chemical Name: RS-246204
- CAS Number: 878451-87-7
- Molecular Formula: C16H13N5O2S
- Molecular Weight: 339.372
- Catalog: Research Areas Cancer
- Create Date: 2019-01-20 14:44:28
- Modify Date: 2024-01-30 13:22:51
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RS-246204 is a R-spondin-1 substitute compound that is able initiate small intestinal organoids without the use of the R-spondin-1 protein.
| Name | RS-246204 |
|---|---|
| Synonyms |
3,7-Dimethyl-8-(2-quinolinylsulfanyl)-3,7-dihydro-1H-purine-2,6-dione
1H-Purine-2,6-dione, 3,7-dihydro-3,7-dimethyl-8-(2-quinolinylthio)- 2H-purin-2-one, 3,7-dihydro-6-hydroxy-3,7-dimethyl-8-(2-quinolinylthio)- |
| Description | RS-246204 is a R-spondin-1 substitute compound that is able initiate small intestinal organoids without the use of the R-spondin-1 protein. |
|---|---|
| Related Catalog | |
| In Vitro | R-spondin-1 protein is added at a high concentration for the initiation and maintenance of the organoids. RS-246204 is a R-spondin-1 substitute that could promote the initial formation and growth of enteroids in the medium without R-spondin-1. The enteroids grown with RS-246204 had a similar differentiation capacity as well as self-renewal capacity as the enteroids grown with R-spondin-1. Furthermore, the RS-246204-derived enteroids could successfully produce the forskolin induced swelling and the organoid based epithelial to mesenchymal transition model[1]. |
| In Vivo | RS-246204 significantly increased BrdU+ cells and body weight in the DSS induced colitis model. These results suggest that RS-246204 activates the R-spondin dependent pathway in Lgr5 stem cells, thereby enhancing proliferation or the anti-apoptotic effect, promoting regeneration of the intestinal epithelium and reducing the disease severity[1]. |
| Animal Admin | Mice[1] Seven-week-old C57Bl/6 mice are given a 2.5% dextran sulfate sodium treatment in drinking water for 5 days. RS-246204 (10 mg/kg in DMSO) is administered intraperitoneally at the end of the DSS exposure. The RS-246204 treatment is repeated every 24 h for 5 days. Daily assessments of disease activity are performed including measuring body weight and evaluating stool consistency. The mice are sacrificed the next day after the last injection of RS-246204. BrdU (50 mg/kg in saline) is administered intraperitoneally 24 hours before sacrifice[1]. |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Molecular Formula | C16H13N5O2S |
| Molecular Weight | 339.372 |
| Exact Mass | 339.079010 |
| LogP | 2.80 |
| Index of Refraction | 1.784 |
| Storage condition | 2-8℃ |