2036044-77-4

2036044-77-4 structure

Name	Porcupine-IN-1
Synonyms	MFCD31630804

Description	Porcupine-IN-1 is potent porcupine inhibitor with an IC50 of 0.5±0.2 nM.
Related Catalog	Signaling Pathways >> Stem Cell/Wnt >> Porcupine Research Areas >> Cancer
Target	IC50: 0.5±0.2 nM (Porcupine inhibitor)[1]
In Vitro	Porcupine is an enzyme that catalyses the addition of palmitoleate to a serine residue in Wnt proteins, a process which is required for the secretion of Wnt proteins. Porcupine-IN-1 is as potent as the clinical compound LGK974 in a cell based STF reporter gene assay. Porcupine-IN-1 potently inhibits the secretion of Wnt3A, therefore is confirmed to be a porcupine inhibitor[1].
In Vivo	Porcupine-IN-1 demonstrates moderate clearance under the treatment of human liver microsomes (57 mL/min/kg) and rat liver microsomes (24 mL/min/kg). It exhibits high clearance when treated with mouse microsomes (109 mL/min/kg)[1].
Cell Assay	HEK293T cells are transfected with pLinbin-Wnt3A plasmid or vehicle control. The HEK293T cells are then treated with or without compounds (Porcupine-IN-1). Western Blot is used after 48 h to analyze both the cell lysis and culture medium[1].
References	[1]. Xu Z, et al. Design, synthesis, and evaluation of novel porcupine inhibitors featuring a fused 3-ring system based on the 'reversed' amide scaffold.

Molecular Formula	C₂₅H₁₉FN₄O
Molecular Weight	410.44