1443530-05-9

1443530-05-9 structure

Name: Rogaratinib
Chemical Name: Rogaratinib
CAS Number: 1443530-05-9
Molecular Formula: C₂₃H₂₆N₆O₃S
Molecular Weight: 466.556
Catalog: Signaling Pathways Protein Tyrosine Kinase/RTK FGFR
Create Date: 2018-10-17 19:35:57
Modify Date: 2024-01-12 23:49:57
Rogaratinib is a potent and selective fibroblast growth factor receptor (FGFR) inhibitor.

Name	Rogaratinib
Synonyms	4-{[4-Amino-6-(methoxymethyl)-5-(7-methoxy-5-methyl-1-benzothiophen-2-yl)pyrrolo[2,1-f][1,2,4]triazin-7-yl]methyl}-2-piperazinone 10372 2-Piperazinone, 4-[[4-amino-6-(methoxymethyl)-5-(7-methoxy-5-methylbenzo[b]thien-2-yl)pyrrolo[2,1-f][1,2,4]triazin-7-yl]methyl]- 98BSN6N516 Rogaratinib UNII:98BSN6N516

Description	Rogaratinib is a potent and selective fibroblast growth factor receptor (FGFR) inhibitor.
Related Catalog	Signaling Pathways >> Protein Tyrosine Kinase/RTK >> FGFR Research Areas >> Cancer
Target	FGFR1 FGFR2 FGFR3 FGFR4
In Vitro	Of the 24 cell lines, 2 FGFR1-amplified lung cancer (LC) cell lines, H1581 and DMS114, show extreme sensitivity to Rogaratinib (BAY1163877) (GI50 values ranging from 36 to 244 nM). Treatment with Rogaratinib results in a significant decrease in colonies formed by H1581P cells, but not by H1581AR and BR cells. Ectopic expression of Met significantly induces resistance to Rogaratinib in MTT assays. Met overexpression induces activation of downstream extracellular signal-regulated kinase 1/2 (ERK1/2) and AKT, which cannot be abrogated by Rogaratinib treatment[1].
Cell Assay	Cells (3000 cells/well) are seeded on 96-well plates at 37°C. After overnight incubation, the cells are treated with Rogaratinib for 72 h. Then, MTT reagent [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] is added to each well and incubated for 4 h at 37°C. MTT solubilization solution/stop mix is added to each well, mixed, and the plates are incubated overnight at 37°C. After measuring the absorbance at 570 nm, the data are graphically displayed[1].
References	[1]. Kim SM, et al. Activation of the Met kinase confers acquired drug resistance in FGFR-targeted lung cancer therapy. Oncogenesis. 2016 Jul 18;5(7):e241.