1384268-04-5
1384268-04-5 structure
- Name: SYP-5
- Chemical Name: SYP-5
- CAS Number: 1384268-04-5
- Molecular Formula: C18H16O3S
- Molecular Weight: 312.383
- Catalog: Signaling Pathways Metabolic Enzyme/Protease HIF/HIF Prolyl-Hydroxylase
- Create Date: 2018-01-11 07:31:56
- Modify Date: 2024-01-10 21:06:08
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SYP-5 is a novel HIF-1 inhibitor, suppresses tumor cells invasion and angiogenesis.
| Name | SYP-5 |
|---|---|
| Synonyms |
(2E)-1-(5-Hydroxy-2,2-dimethyl-2H-chromen-6-yl)-3-(2-thienyl)-2-propen-1-one
2-Propen-1-one, 1-(5-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl)-3-(2-thienyl)-, (2E)- |
| Description | SYP-5 is a novel HIF-1 inhibitor, suppresses tumor cells invasion and angiogenesis. |
|---|---|
| Related Catalog | |
| Target |
HIF[1] |
| In Vitro | SYP-5 inhibits hypoxia-induced upregulation of HIF-1. SYP-5 inhibits HIF-1 and downstream gene expression in Hep3B and Bcap37 cells. SYP-5 inhibits tumor cell migration and invasion, as well as tumor angiogenesis, which are mediated by suppressing PI3K/AKT- and MAPK/ERK-dependent HIF-1 pathway. The proteins of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMP)-2 that are targets of HIF-1, are down-regulated by SYP-5. SYP-5 displays significant inhibition on hypoxia-induced overexpression of VEGF and MMP2 in both cell lines. In the tube formation assay, SYP-5 suppresses angiogenesis induced by hypoxia and VEGF in vitro. SYP-5 also retards the Hep3B and Bcap37 cells migration and invasion induced by hypoxia and FBS. SYP-5 specifically inhibits hypoxic induction of luciferase expression in U251-HRE but not in U251-pGL3[1]. |
| Cell Assay | The cells (1×105 cells/mL) are seeded into 96-well culture plates. After overnight ncubation, the cells are treated with various concentrations of SYP-5 (2, 10, 50 μM) for 24 h. Then 10μLMTT) solution (2.5 mg/mL in PBS) is added to each well, and the plates are incubated for additional 4 h at 37°C. After centrifugation (2500 rpm, 10 min), the medium containing MTT is aspirated, and 100 μL DMSO is added. The optical density of each well is measured at 570 nm with a SpectraMax Paradigm Reader[1]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 502.0±50.0 °C at 760 mmHg |
| Molecular Formula | C18H16O3S |
| Molecular Weight | 312.383 |
| Flash Point | 257.4±30.1 °C |
| Exact Mass | 312.082001 |
| LogP | 6.34 |
| Vapour Pressure | 0.0±1.3 mmHg at 25°C |
| Index of Refraction | 1.649 |
| Storage condition | 2-8℃ |