DC Chemicals Limited
China
Product Name: Adaptavir
Price: $Inquiry/100mg $Inquiry/250mg $Inquiry/500mg
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

Nanjing Peptide Biotech Ltd
China
Product Name: dapta
Price: ￥Inquiry/10g ￥Inquiry/100g ￥Inquiry/1kg ￥Inquiry/10kg
Purity: 98.0%
Stocking Period: 7 Day
Contact: jom

106362-34-9

106362-34-9 structure

106362-34-9 structure

Name: (D-Ala1)-Peptide T amide
Chemical Name: dapta
CAS Number: 106362-34-9
Molecular Formula: C₃₅H₅₆N₁₀O₁₅
Molecular Weight: 856.87700
Catalog: Peptides
Create Date: 2016-01-08 22:46:10
Modify Date: 2024-01-02 15:51:38
DAPTA is a synthetic peptide, functions as a viral entry inhibitor by targeting selectively CCR5, and shows potent anti-HIV activities. Sequence: Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-NH2.

Name	dapta
Synonyms	L-ThreoninaMide,D-alanyl-L-seryl-L-threonyl-L-threonyl-L-threonyl-L-asparaginyl-L-tyrosyl D-ALA-SER-THR-THR-THR-ASN-TYR-THR-NH2 D-Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr amide acetate salt D-Ala-L-Ser-L-Thr-L-Thr-L-Thr-L-Asn-L-Tyr-L-Thr-NH2 H-D-ALA-SER-THR-THR-THR-ASN-TYR-THR-NH2 Octapeptide T D-ala-ser-thr-thr-thr-asn-tyr-thr amide acetate (D-ALA1)-PEPTIDE T AMIDE Adaptavir

Description	DAPTA is a synthetic peptide, functions as a viral entry inhibitor by targeting selectively CCR5, and shows potent anti-HIV activities. Sequence: Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-NH2.
Related Catalog	Signaling Pathways >> Anti-infection >> HIV Research Areas >> Infection Peptides
Target	gp120BaL/sCD4-CCR5:55 pM (IC50, in Cf2Th/synR5 cells) gp120CMCM235/sCD4-CCR5:0.32 nM (IC50, in Cf2Th/synR5 cells)
In Vitro	DAPTA (1 nM) inhibits HIV-1 replication in monocytes/macrophages (M/M) by >90%. DAPTA blocks HIV entry and prevents HIV-1 infection. DAPTA reduces CCR5 mAb binding in human primary macrophages. DAPTA potently blocks R5 gp120-mediated neuronal apoptosis. DAPTA is even more potent in preventing neuronal apoptosis than the CCR5 antagonist TAK-779[1]. DAPTA potently inhibits specific CD4-dependent binding of gp120 Bal (IC50 = 0.06 nM) and CM235 (IC50 = 0.32 nM) to CCR5. DAPTA (1 nM) blocks formation of the gp120/sCD4 complex with CCR5. DAPTA inhibits the binding of gp120BaL/sCD4 to CCR5 (Cf2Th/synR5) cells with IC50 of 55 ± 0.08 pM[2].
Kinase Assay	A novel FITC-labeled tracer from soluble gp120 proteins (25 g/mL) is prepared using a Fluorescent protein labeling kit, according to the manufacture's instructions. Uncoupled FLUOS is removed by Sephadex G-10 column filtration. The molar ratio between FLUOS-labeling molecules and protein is from 3.5 to 4.5 fluorescence molecules per molecule of gp120. The concentration of fluorescent-labeled proteins is measured by Bradford assay and Western blotting by using calibrating amounts of soluble molecules with known concentration. Binding assays are performed in binding buffer, in final volume 100l. Binding is carried out for 1 h at 37°C in 96-well filter plates. Unbound-labeled proteins are removed by rapid vacuum filtration and ishing using a 96-well plates manifold. Each binding mix is washed five times with 0.2 mL (total volume of 1.0 mL/well) cold ishing buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM CaCl2). Filters are counted with a fluorescent plate reader at 495/530 nm.
References	[1]. Pollicita M, et al. Profound anti-HIV-1 activity of DAPTA in monocytes/macrophages and inhibition of CCR5-mediated apoptosis in neuronal cells. Antivir Chem Chemother. 2007;18(5):285-95. [2]. Polianova MT, et al. Chemokine receptor-5 (CCR5) is a receptor for the HIV entry inhibitor peptide T (DAPTA). Antiviral Res. 2005 Aug;67(2):83-92.

Density	1.415g/cm3
Boiling Point	1514.3ºC at 760mmHg
Molecular Formula	C₃₅H₅₆N₁₀O₁₅
Molecular Weight	856.87700
Flash Point	869.6ºC
Exact Mass	856.39300
PSA	437.28000
Vapour Pressure	0mmHg at 25°C
Index of Refraction	1.596
Storage condition	-20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3