STAT3-IN-13
Names
[ CAS No. ]:
2248552-86-3
[ Name ]:
STAT3-IN-13
Biological Activity
[Description]:
STAT3-IN-13 (compound 6f) is a potent STAT3 inhibitor. STAT3-IN-13 has anti-proliferative effects and binds to the STAT3 SH2 domain with a KD of 0.46 μM. STAT3-IN-13 inhibits the phosphorylation of STAT3 Y705 and downstream target gene expression. STAT3-IN-13 induces apoptosis in vitro and suppresses the growth and metastasis of tumor in vivo. STAT3-IN-13 can be used for cancer research[1].
[Related Catalog]:
[In Vitro]
STAT3-IN-13 (compound 6f) (48 hours) has anti-proliferative activity with IC50 values of 0.25, 0.11 and 0.55 μM for 143B, HOS and MG63 cells, respectively[1]. STAT3-IN-13 (compound 6f) (0.001-100 μM) binds to STAT3 and interacts with STAT3586−685 in a concentration dependent manner with a KD of 0.96 μM[1]. STAT3-IN-13 (compound 6f) (0-1.0 μM; 24 hours; 143B and HOS cells) inhibits STAT3 Y705 phosphorylation and suppresses STAT3 in tumor cells[1]. STAT3-IN-13 (compound 6f) (0-1.0 μM; 48 hours; 143B cells) induces apoptosis in a dose-dependent manner[1]. Apoptosis Analysis[1] Cell Line: 143B cells Concentration: 0, 0.2, 0.5, and 1 μM Incubation Time: 48 hours Result: Increased the percentage of apoptosis rates of 3.4%, 8.7%, 10.7%, and 23.3% at 0, 0.2, 0.5 and 1 μM, respectively. Western Blot Analysis[1] Cell Line: 143B and HOS cells Concentration: 0, 0.2, 0.5 and 1.0 μM Incubation Time: 24 hours Result: Inhibited STAT3 Y705 phosphorylation and downstream target gene expression including Bcl-2 and VEGF, retained good antitumor activities against control tumor cells without STAT3 knockdown.
[In Vivo]
STAT3-IN-13 (compound 6f) (10-20 mg/kg; i.p.; twice daily, for 4 weeks; nude mice) blocks osteosarcoma growth and metastasis in vivo[1]. Animal Model: Nude mice Dosage: 10 and 20 mg/kg Administration: Intraperitoneal injection; twice daily, for 4 weeks Result: Suppressed tumor weight at a dose of 10 mg/kg.
[References]
Chemical & Physical Properties
[ Molecular Formula ]:
C21H20N6O3S
[ Molecular Weight ]:
436.49