OVA Peptide 257-264

Names

[ CAS No. ]:
138831-86-4

[ Name ]:
OVA Peptide 257-264

[Synonym ]:
Ovalbumin (257-264) chicken
OVALBUMIN(257-264) ANTIGEN PEPTIDE
OVA PEPTIDE (257-264)
SIINFEKL
CHICKEN OVALBUMIN FRAGMENT (257-264)
SER-ILE-ILE-ASN-PHE-GLU-LYS-LEU
OVA (257-264) (CHICKEN)
H2N-SIINFEKL-OH
OVA (257-264)
OVALBUMIN (257-264) (CHICKEN)

Biological Activity

[Description]:

OVA Peptide (257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide from ovalbumin presented by the class I MHC molecule, H-2Kb.

[Related Catalog]:

Signaling Pathways >> Others >> Others
Research Areas >> Others
Peptides

[In Vitro]

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide (257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide (257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide (257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide (257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

[Cell Assay]

TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide (257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

[References]

[1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.


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Chemical & Physical Properties

[ Molecular Formula ]:
C45H74N10O13

[ Molecular Weight ]:
963.12800

[ Exact Mass ]:
962.54400

[ PSA ]:
393.66000

[ LogP ]:
2.87160

[ Storage condition ]:
2-8℃

Related Compounds