β-Apopicropodophyllin

β-Apopicropodophyllin is a nature product that could be isolated from Hyptis wticillata.β-Apopicropodophyllin induces apoptosis by inducing microtubule disruption, DNA damage, cell cycle arrest and ER stress. β-Apopicropodophyllin can be used in research of cancer[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

β-Apopicropodophyllin (0-100 nM; 48和72 h) 对 A549、NCI-H1299 和 NCI-460 细胞系具有抗癌活性，IC50 值分别为 16.9、13.1 和 17.1 nM [1]。 β-Apopicropodophyllin (0-20 nM; 24和48小时; A549, NCI-H1299 和 NCI-460细胞系) 破坏细胞内微管的聚合[1]。 β-Apopicropodophyllin (0-20 nM; 0-24 h; A549, NCI-H1299 和 NCI-460细胞株) 通过激活 ATM 诱导 DNA 损伤，将细胞周期阻滞在 G2/M 期，诱导内质网应激[1]。 β- apoopicropodophyllin (15 和 20 nM; 48小时; A549, NCI-H1299 和 NCI-460 细胞系) 诱导体外凋亡细胞死亡[1]。 Cell Viability Assay[1] Cell Line: A549, NCI-H1299 and NCI-460 cells Concentration: 0-100 nM Incubation Time: 48 and 72 hors Result: Inhibited cell growth in a dose-dependent manner. Apoptosis Analysis[1] Cell Line: A549, NCI-H1299 and NCI-460 cells Concentration: 15 and 20 nM Incubation Time: 48 h Result: Increased the percentage of apoptotic death in A549, NCI-H1299 and NCI-H460 cell groups in a time-dependent manner. Cell Cycle Analysis[1] Cell Line: A549, NCI-H1299 and NCI-460 cells Concentration: 0-20 nM Incubation Time: 8 and 16 h Result: Arrested cell cycle in G2/M phase. Western Blot Analysis[1] Cell Line: A549, NCI-H1299 and NCI-460 cells Concentration: 0-20 nM Incubation Time: 24 and 48 hors Result: Decreased the level of insoluble protein containing polymeric microtubules in a time-dependent manner in non-small cell lung cancer cell lines. Western Blot Analysis[1] Cell Line: A549, NCI-H1299 and NCI-460 cells Concentration: 0-20 nM Incubation Time: 24 h Result: Increased phosphorylated ATM and γ-H2AX levels in a dose-dependent manner.

β-Apopicropodophyllin (1 和 5 mg/kg; 瘤内注射; 异种移植裸鼠) 以剂量依赖性方式延缓肿瘤生长[1]。 Animal Model: nude mice with xenografts[1] Dosage: 1 and 5 mg/kg Administration: intra-tumorous injection Result: Inhibited tumor growth of NSCLC xenografts in nude mice.

[1]. Kim JY, et, al. A novel anti-cancer role of β-apopicropodophyllin against non-small cell lung cancer cells. Toxicol Appl Pharmacol. 2018 Oct 15;357:39-49.