(-)-Pinoresinol 4-O-glucoside

(-)-Pinoresinol 4-O-glucoside ((-)-Pinoresinol 4-O-β-D-glucopyranoside) is a potent and orally active α-glucosidase inhibitor with an IC50 value of 48.13 µM. (-)-Pinoresinol 4-O-glucoside increases cell migration and early differentiation of pre-osteoblasts. (-)-Pinoresinol 4-O-glucoside increases protein level of BMP2, p-Smad1/5/8, RUNX2. (-)-Pinoresinol 4-O-glucoside attenuates oxidative stress, hyperglycemia and hepatic toxicity. (-)-Pinoresinol 4-O-glucoside has the potential for the research of osteoporosis and periodontal disease[1][2].

Research Areas >> Metabolic Disease

IC50: 48.13 µM (α-Glucosidase)[1]

(-)-Pinoresinol 4-O-glucoside (0、10、30 µM；24 小时) 在含有 50 μg/mL 的成骨补充培养基 (OS) 中增加前成骨细胞分化过程中的细胞迁移[1]< /sup>. (-)-Pinoresinol 4-O-glucoside (10、30 µM；7 天) 在前成骨细胞分化过程中增加早期分化并增加矿化结节的形成[1]。 (-)-Pinoresinol 4-O-glucoside (10、30 µM；3 天) 增加前成骨细胞中 BMP2、ALP、OCN mRNA 水平的表达[1]。 (-)-Pinoresinol 4-O-glucoside (10、30 µM；3 天) 增加 BMP2、p-Smad1/5/8、RUNX2 的蛋白表达水平[1]。 RT-PCR[1] Cell Line: pre-osteoblasts Concentration: 10, 30 µM Incubation Time: 3 days Result: Upregulated the mRNA level of BMP2 and its target osteoblast genes, ALP and osteocalcin (OCN). Western Blot Analysis[1] Cell Line: pre-osteoblasts Concentration: 10, 30 µM Incubation Time: 3 days Result: Enhanced protein level of BMP2, followed by the phosphorylation of Smad1/5/8 and the expression of RUNX2.

(-)-Pinoresinol 4-O-glucoside (50 mg/kg；口服；20 天) 减轻小鼠的氧化应激、高血糖和肝毒性[2]。 Animal Model: 27-30 g, Male Swiss albino mice[2] Dosage: 50 mg/kg Administration: P.o.; twenty days Result: Exhibited a hepatoprotective activity in vivo as it lowered AST and ALT levels, caused a prominent decline in serum glucose level by 37.83% in streptozotocin-treated mice with promising elevation in insulin level of 25.37%.

[1]. Park KR, et al. Effects of PIN on Osteoblast Differentiation and Matrix Mineralization through Runt-Related Transcription Factor. Int J Mol Sci. 2020 Dec 16;21(24):9579.  

[2]. Youssef FS, et al. Pinoresinol-4-O-β-D-glucopyranoside: a lignan from prunes (Prunus domestica) attenuates oxidative stress, hyperglycaemia and hepatic toxicity in vitro and in vivo. J Pharm Pharmacol. 2020 Dec;72(12):1830-1839.