bursehernin

Bursehernin (Methylpluviatolide) is an antitumor agent. Bursehernin induces Apoptosis and cell cycle arrest at G2/M phase. Bursehernin shows anti-proliferative activity[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Bursehernin (4.3 µM for MCF-7 cells, 3.7 µM for KKU-M213 cells; 4, 48, 72 h) 以时间依赖的方式诱导细胞凋亡和细胞周期停滞在G2/M期 [1]。 Cell Proliferation Assay [1] Cell Line: MCF-7, MDA-MB-468, MDA-MB-231, HT-29, KKU-M213, KKU-K100, KKU-M055, L-929, MMNK-1 cells Concentration: 0-100 µM Incubation Time: 72 h Result: Showed anti-proliferative activity with IC50s of 11.96, 8.24, 14.26, 47.53, 3.70, 12.38, 17.38, 26.36, 7.45 µM for MCF-7, MDA-MB-468, MDA-MB-231, HT-29, KKU-M213, KKU-K100, KKU-M055, L-929, MMNK-1 cells, respectively. Cell Cycle Analysis [1] Cell Line: MCF-7, KKU-M213 cells Concentration: 4.3 µM for MCF-7 cells, 3.7 µM for KKU-M213 cells Incubation Time: 24, 48, 72 h Result: Induced cell cycle arrest at G2/M phase. Western Blot Analysis [1] Cell Line: MCF-7, KKU-M213 cells Concentration: 4.3 µM for MCF-7 cells, 3.7 µM for KKU-M213 cells Incubation Time: 24, 48, 72 h Result: Decreased the expression of topoisomerase II, STAT 3, cyclin D1, and p21. Apoptosis Analysis [1] Cell Line: MCF-7 cells Concentration: 0, 2.15, 4.30, 8.60 µM Incubation Time: 24, 48, 72, 96 h Result: Induced apoptosis in a time- and dose-dependent manner.

[1]. Rattanaburee T, et al. Anticancer activity of synthetic (±)-kusunokinin and its derivative (±)-bursehernin on human cancer cell lines. Biomed Pharmacother. 2019 Sep;117:109115.  

[2]. McDoniel PB, et al. Antitumor activity of Bursera schlechtendalii (burseraceae): isolation and structure determination of two new lignans. J Pharm Sci. 1972 Dec;61(12):1992-4.