α-Zearalenol

α-Zearalenol is a Mycotoxin with high affinity for the estrogen receptors (ER),α-Zearalenol is the derivative of zearalenone (ZEN), causes reproductive disorders in animals, due to its xenoestrogenic effects[1].

Signaling Pathways >> Others >> Estrogen Receptor/ERR

Research Areas >> Inflammation/Immunology

α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) shows the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells with an IC50 of 12.5 μM[1]. α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells[2]. α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) increases MCF7 cell population in the S phase and in the G2/M phase, no visible change in cell cycle phases is observed in MDA-MB231 cells[2]. α-Zearalenol (α-zol) (1-10 μM; 24 hours) reduces the expression of TNF-α, α-ZOL combines with β-ZOL shows an antagonistic effect on inflammation for IL-1β and act synergic for IL-8 at high toxin concentrations[2]. Cell Proliferation Assay[1] Cell Line: MFC-7 cells Concentration: 6.25 µM-25 µM Incubation Time: 24 hours Result: Observed the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells. Cell Viability Assay[2] Cell Line: Breast cancer cell lines: MCF-7 and MDA-MB231 Concentration: 0.001 μM, 0.1 μM and 10 μM Incubation Time: 24 hours, 48 hours, 72 hours Result: Modified the cell growth in two breast cancer cell lines. Cell Cycle Analysis[2] Cell Line: MCF7 cells, MDA-MB231 cells Concentration: 0.001 μM, 0.1 μM and 10 μM Incubation Time: 72 hours Result: Observed an increase of cells in cell cycle in MCF7 cells. RT-PCR[2] Cell Line: HepG2 cells Concentration: 1 μM, 5 μM, 10 μM Incubation Time: 72 hours Result: Induced a decrease of the expression of TNF-α, IL-1β and IL-8, that was in most cases statistically significant for the higher doses.

[1]. .Tatay E, et al. Estrogenic activity of zearalenone, α-zearalenol and β-zearalenol assessed using the E-screen assay in MCF-7 cells. Toxicol Mech Methods. 2018 May;28(4):239-242.

[2]. Marin DE, et al. Cytotoxic and inflammatory effects of individual and combined exposure of HepG2 cells to zearalenone and its metabolites. Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug;392(8):937-947.

[3]. Nittoli AC, et al. Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. Metabolomics. 2018 Feb 14;14(3):33.