RH01386

RH01386 is a small molecule that protect pancreatic β cells against endoplasmic reticulum stress-induced cell death; increases the survival of human primary β cells and rodent β cell lines subjected to ER stressors including palmitate; also restores ER stress-impaired glucose-stimulated insulin secretion responses.

Signaling Pathways >> Others >> Others

Research Areas >> Metabolic Disease

ER stress-induced β cell dysfunction[1]

RH01386 (270 nM-70 μM; 72 hours) is confirmed to increase ATP levels in βTC6 cells treated with (tunicamycin) Tm, exhibits a EC50 value of 1.894 μM[1]. RH01386 (270 nM-70 μM; 72 hours) dose-dependently inhibits (tunicamycin) Tm-induced cell death, and inhibits the activity of caspase-3, a downstream effector of the apoptotic pathway in βTC6 cells[1]. Cell Viability Assay[1] Cell Line: βTC6 cells Concentration: 270 nM-70 μM Incubation Time: 72 hours Result: Inhibited ER stress-induced death of βTC6 cells in a dose-dependent manner. Apoptosis Analysis[1] Cell Line: βTC6 cells Concentration: 270 nM-70 μM Incubation Time: 72 hours Result: Protected βTC6 cells from Tm-induced cell death.

[1]. Tran K, et al. Identification of small molecules that protect pancreatic β cells against endoplasmic reticulum stress-induced cell death.ACS Chem Biol. 2014 Dec 19;9(12):2796-806.